Results 161 to 170 of about 3,677,140 (187)

Purification and characterization from bovine brain membranes of a GTP-binding protein with a Mr of 21,000, ADP-ribosylated by an ADP-ribosyltransferase contaminated in botulinum toxin type C1 4- Identification as the rhoA gene product

Molecular Brain Research, 1990
Abstract We have previously shown that there are multiple GTP-binding proteins (G proteins) with M r values of about 20,000 in bovine brain membranes and identified one G protein with a M r of 20,000 as the rho gene product. We have also shown that this rho gene product is ADP-ribosylated by an ADP-ribosyltransferase contaminated in ...
Jun Kondo, Masahiko Hoshijima, J Kondo   +2 more
exaly   +2 more sources

Differential expression of the small GTP-binding proteins RhoA, RhoB, Cdc42u and Cdc42b in developing rat neocortex

Molecular Brain Research, 1999
Studies with cultured cells indicate that small GTPases of the Rho family may be involved in cell proliferation, differentiation, as well as migration. Therefore, we have studied the expression of four members of this protein family, i.e., RhoA, RhoB, the ubiquitous Cdc42u, and brain specific Cdc42b, during the embryonic and early postnatal development
Olenik, Claudia, Aktories, Klaus, Meyer, Dieter K.   +2 more
openaire   +3 more sources

The small GTP-binding protein RhoA regulates serotonin-induced Na+-current response in the neurons of Aplysia

Neuroscience Research, 2004
Application of serotonin (5-HT) induces a slow inward current response in identified neurons of Aplysia ganglia under voltage clamp. The 5-HT-induced current response was depressed in Na+-free media, but augmented in Ca2+-free media, and unaffected by a change in external K+.
Satoshi, Kawasaki, Shingo, Kimura, Reiko, Fujita, Kazuhiko, Sasaki   +3 more
openaire   +2 more sources

Gene expression of the small GTP-binding proteins RhoA, RhoB, Rac1, and Cdc42 in adult rat brain

Molecular Brain Research, 1997
GTPases of the Rho subfamily, i.e. Rho, Rac and Cdc42, are molecular switches in various signaling pathways. Best characterized are their functions in the regulation of the actin cytoskeleton. In neuronal cell lines they are involved in the mechanisms leading to synapse formation and plasticity.
Olenik, Claudia, Barth, Holger, Just, Ingo, Aktories, Klaus, Meyer, Dieter K.   +4 more
openaire   +3 more sources

RANTES Activation of Phospholipase D in Jurkat T Cells: Requirement of GTP-Binding Proteins ARF and RhoA

The Journal of Immunology, 1998
Abstract The chemokine RANTES is a potent agonist of T cell activation. In an investigation of signal-transduction events activated by this chemokine, we have shown that RANTES stimulates dose-dependent phospholipase D (PLD) activity in Jurkat cells.
K B, Bacon, T J, Schall, D J, Dairaghi
openaire   +2 more sources

Distribution of the small molecular weight GTP-binding proteins Rac1, Cdc42, RhoA and RhoB in the developing chick retina

Journal of Neurocytology, 2002
Immunohistochemistry was used to determine the distribution of Rac1, Cdc42, RhoA and RhoB GTPases during development of the chick retina. All proteins appear as early as embryonic day 5 (E5) in cells of the vitreal margin, E7-8 in cells of the inner third of the inner nuclear layer and E9-10 in photoreceptors.
Andréa Silveira, Santos-Bredariol, Marinilce Fagundes, Santos, Dânia Emi, Hamassaki-Britto   +2 more
openaire   +2 more sources

Activation of Membrane-Bound Phospholipase D by Protein Kinase C in HL60 Cells: Synergistic Action of a Small GTP-Binding Protein RhoA

Biochemical and Biophysical Research Communications, 1995
Regulation of phospholipase D (PLD) activation by protein kinase C (PKC) was studied in membranes isolated from human promyelocytic leukemia HL60 cells. The activation of membrane-bound PLD by PKC partially purified from rat brain was most effectively induced with phorbol 12-myristate 13-acetate (PMA) and Ca2+ (1 microM) which caused translocation of ...
K, Ohguchi, Y, Banno, S, Nakashima, Y, Nozawa   +3 more
openaire   +2 more sources

Identification of a major GTP-binding protein in bovine aortic smooth muscle cytosol as the A gene product

Biochemical and Biophysical Research Communications, 1990
In bovine aortic smooth muscle, about 50% of total GTP-binding activity was present in the cytosol fraction. A major GTP-binding protein (G protein) with a Mr value of about 21,000 (21K G) in this fraction was purified to near homogeneity and characterized.
Y, Kawahara, M, Kawata, M, Sunako, S, Araki, M, Koide, T, Tsuda, H, Fukuzaki, Y, Takai   +7 more
openaire   +2 more sources

Overexpression of small GTP-binding protein RhoA promotes invasion of tumor cells.

Cancer research, 1999
Adhesion of tumor cells to host cell layers and subsequent migration are pivotal steps in cancer invasion and metastasis. The small GTP-binding protein RhoA controls cell adhesion and motility through organization of the actin cytoskeleton and regulation of actomyosin contractility.
K, Yoshioka, S, Nakamori, K, Itoh
openaire   +1 more source

Effects of the Low-Molecular-Weight GTP-Binding Protein RhoAon Calcium Waves in Xenopus Oocytes

Biochemical and Biophysical Research Communications, 1996
In normal Xenopus oocytes, injection of high concentrations of inositol-1,4,5-trisposphate (IP3) always elicited a Ca2+ wave, which propagated from the injection site toward the opposite side of the oocyte. In oocytes injected with C3 toxin, a specific blocker of RhoA, a similar Ca2+ wave was still observed, but propagation of the wave appeared to be ...
openaire   +2 more sources