Engineering Rhodosporidium toruloides for limonene production [PDF]
Biotechnology for Biofuels, 2021 Background Limonene is a widely used monoterpene in the production of food, pharmaceuticals, biofuels, etc. The objective of this work was to engineer Rhodosporidium toruloides as a cell factory for the production of limonene.
Sasa Liu +7 more
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Mining and application of constitutive promoters from Rhodosporidium toruloides [PDF]
AMB Express, 2023 Rhodosporidium toruloides is an oleaginous yeast under development with promising industrial applications. Since promoters of different strengths have been demonstrated as an efficient strategy to fine-tune gene expression in synthetic biology, a set of ...
Xiao Guo +4 more
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Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in Rhodosporidium toruloides [PDF]
Journal of Fungi, 2021 Oleaginous yeast Rhodosporidium toruloides has great biotechnological potential and scientific interest, yet the molecular rationale of its cellular behavior to carbon and nitrogen ratios with concurrent lipid agglomeration remains elusive.
Farha Deeba +3 more
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Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization in Rhodosporidium toruloides [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 An oleaginous yeast Rhodosporidium toruloides is a promising host for converting lignocellulosic biomass to bioproducts and biofuels. In this work, we performed multi-omics analysis of lignocellulosic carbon utilization in R. toruloides and reconstructed
Joonhoon Kim +30 more
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Genetic manipulation of the interconversion between diacylglycerols and triacylglycerols in Rhodosporidium toruloides [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 The basidiomycetous yeast Rhodosporidium toruloides (R. toruloides) is an excellent producer for neutral lipids, including triacylglycerols (TAG). Partially because genetic tools for this yeast were less developed, limited efforts were shown to explore ...
Yue Zhang +13 more
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Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Oleaginous yeasts are a promising candidate for the sustainable conversion of lignocellulosic feedstocks into fuels and chemicals, but their growth on these substrates can be inhibited as a result of upstream pretreatment and enzymatic ...
William Woodruff +4 more
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Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction [PDF]
Bioresources and Bioprocessing, 2023 Lipids produced by oleaginous yeasts are considered as sustainable sources for the production of biofuels and oleochemicals. The red yeast Rhodosporidium toruloides can accumulate lipids to over 70% of its dry cell mass. To facilitate lipid extraction, a
Shiyu Liang +4 more
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Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts [PDF]
Microbial Cell Factories, 2023 Efficient conversion of pentose sugars remains a significant barrier to the replacement of petroleum-derived chemicals with plant biomass-derived bioproducts. While the oleaginous yeast Rhodosporidium toruloides (also known as Rhodotorula toruloides) has
Samuel T. Coradetti +11 more
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The use of flow cytometry to assess Rhodosporidium toruloides NCYC 921 performance for lipid production using Miscanthus sp. hydrolysates [PDF]
Biotechnology Reports, 2021 The yeast Rhodosporidium toruloides NCYC 921 was used for lipid production, using Miscanthus biomass hydrolysate as carbon source. The hydrolysate was obtained by enzymatic hydrolysis of Miscanthus biomass (at high solids loading) previously subjected to
Joana Alves Martins +6 more
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Mining novel cis-regulatory elements from the emergent host Rhodosporidium toruloides using transcriptomic data [PDF]
Frontiers in Microbiology, 2023 The demand for robust microbial cell factories that produce valuable biomaterials while resisting stresses imposed by current bioprocesses is rapidly growing.
Luísa Czamanski Nora +5 more
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