Results 121 to 130 of about 156,401 (384)

Ribonuclease A can be transformed into a dimeric ribonuclease with antitumor activity

Journal of Biological Chemistry, 1994
A cDNA coding for bovine pancreatic RNase A was mutagenized to insert a proline, a leucine, and 2 cysteine residues, i.e. the residues present at corresponding positions in the subunit of seminal RNase, the only dimeric RNase of the pancreatic-type superfamily.
DI DONATO, ALBERTO, CAFARO, VALERIA, D'ALESSIO, GIUSEPPE   +2 more
openaire   +5 more sources

Iron‐dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP‐1 monocytes

FEBS Open Bio, EarlyView.
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník   +4 more
wiley   +1 more source

A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. [PDF]

, 2015
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a
Chen, Qiu-Xia, Urayama, Shiro, Wang, Wei-Peng, Yu, Ai-Ming, Zeng, Su   +4 more
core   +2 more sources

The major component in schistosome eggs responsible for conditioning dendritic cells for Th2 polarization is a T2 ribonuclease (omega-1)

Journal of Experimental Medicine, 2009
Schistosoma mansoni eggs contain factors that trigger potent Th2 responses in vivo and condition mouse dendritic cells (DCs) to promote Th2 lymphocyte differentiation.
S. Steinfelder, J. Andersen, J. Cannons, C. Feng, M. Joshi, D. Dwyer, P. Caspar, P. Schwartzberg, A. Sher, Dragana Jankovic   +9 more
semanticscholar   +1 more source

Profiling the effect of low frequency mechanical vibration on the metabolic and oxidative stress responses of A431 carcinoma

FEBS Open Bio, EarlyView.
20 Hz mechanical vibration induced A431 cancer cells' apoptosis without such effect on other healthy cell lines of L929 and C2C12. Lowered glucose consumption is observed specifically in A431. The expressions of ROS, HMGB1, and HSP1 levels initially increase and subsequently decrease in the cancer cell line, as opposed to L929 and C2C12, which ...
Wresti L. Anggayasti, Chikahiro Imashiro, Takashi Morikura, Shogo Miyata, Akira Funahashi, Kenjiro Takemura   +5 more
wiley   +1 more source

Human mitochondrial degradosome prevents harmful mitochondrial R loops and mitochondrial genome instability [PDF]

, 2018
R loops are nucleic acid structures comprising an DNA-RNA hybrid and a displaced single-stranded DNA. These structures may occur transiently during transcription, playing essential biological functions. However, persistent R loops may become pathological
Aguilera López, Andrés, Pérez de Camino Cantos, Dolores, Silva, Sonia   +2 more
core   +1 more source

Ribonuclease S dynamics measured using a nitrile label with 2D IR vibrational echo spectroscopy.

Journal of Physical Chemistry B, 2012
A nitrile-labeled amino acid, p-cyanophenylalanine, is introduced near the active site of the semisynthetic enzyme ribonuclease S to serve as a probe of protein dynamics and fluctuations.
Sayan Bagchi, S. Boxer, M. Fayer
semanticscholar   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Adenovirus-mediated hPNPase(old-35) gene transfer as a therapeutic strategy for neuroblastoma [PDF]

, 2009
Current treatment options for neuroblastoma fail to eradicate the disease in the majority of high-risk patients, clearly mandating development of innovative therapeutic strategies. Gene therapy represents a promising approach for reversing the neoplastic
Ardelt, Arnold, Beck, Benito, Brodeur, Chan, Cinatl, Cinatl, D'Alessio, Hellemans, Holmes, Jasty, Kotchetkov, Lebedeva, Lebedeva, Leszczyniecka, Leszczyniecka, Maris, McConville, Michaelis, Pattyn, Pattyn, Ross, Sarkar, Sarkar, Sarkar, Sarkar, Sarkar, Sarkar, Su, Tweddle, Van Maerken, Vandesompele, Vandesompele, Vasandani, Vasandani, Youle, Young   +37 more
core   +2 more sources

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source