Results 121 to 130 of about 5,657 (149)

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

Cell, 1985
Two different experimental approaches have provided evidence that both U2 and U1 snRNPs function in pre-mRNA splicing. When the U2 snRNPs in a nuclear extract are selectively degraded using ribonuclease H and either of two deoxyoligonucleotides complementary to U2 RNA, splicing activity is abolished. Mixing an extract in which U2 has been degraded with
D L, Black, B, Chabot, J A, Steitz
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U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

Cell, 1986
The requirement for individual U RNAs in splicing and polyadenylation was investigated using oligonucleotide-directed cleavage of snRNAs in in vitro processing extracts. Cleavage of U1, U2, or U4 RNA inhibited splicing but not polyadenylation of short precursor RNAs.
S M, Berget, B L, Robberson
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Ribonucleoprotein particle assembly and modification of U2 small nuclear RNA containing 5-fluorouridine

Biochemistry, 1993
An in vitro assembly/modification system was used to study the effect of 5-fluorouridine (5-FU) incorporation on the biosynthesis of the U2 small nuclear ribonucleoprotein particle (U2 snRNP). Labeled U2 RNAs were transcribed in vitro with 5-fluoro-UTP either partially supplementing or completely replacing UTP during synthesis.
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Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

Cell, 1985
We have identified six distinct factors necessary for pre-mRNA splicing in vitro by selective inactivation and complementation studies, and by fractionation procedures. Splicing factor 1 (SF1) is sensitive to micrococcal nuclease, and appears to consist of at least U1 and U2 snRNPs, since splicing is inhibited when the 5' termini of U1 and U2 snRNAs ...
A R, Krainer, T, Maniatis
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Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

Science, 1986
The splicing process, which removes intervening sequences from messenger RNA (mRNA) precursors is essential to gene expression in eukaryotic cells. This site-specific process requires precise sequence recognition at the boundaries of an intervening sequence, but the mechanism of this recognition is not understood.
P J, Grabowski, P A, Sharp
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Transcription-dependent localization of U1 and U2 small nuclear ribonucleoproteins at major sites of gene activity in polytene chromosomes

Journal of Molecular Biology, 1984
The location and dynamics of small nuclear ribonucleoproteins (snRNPs) were studied in salivary gland polytene chromosomes of Chironomus tentans by immunofluorescence with specific snRNP antibodies. Monoclonal antibody against the snRNP Sm antigens reacted at all sites of transcription (puffs and Balbiani rings).
H, Sass, T, Pederson
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Interaction of Small Nuclear Ribonucleoproteins with Simian Virus 40 in CV-1 Cells: Is U2 snRNA Involved in Regulating Replication?

DNA, 1984
The small nuclear RNAs (snRNAs) in African Green Monkey kidney cells (CV-1 cells) were examined by polyacrylamide gel electrophoresis. Methodology was developed to improve their extraction from enriched fractions. Cellular fractionation studies and subsequent analysis of these RNAs indicate that they are tightly associated with chromatin.
J F, Savouret, G, Cathala, N L, Eberhardt, W L, Miller, J D, Baxter   +4 more
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Ribonucleoprotein organization of eukaryotic RNA. XXXII. U2 small nuclear RNA precursors and their accurate 3' processing in vitro as ribonucleoprotein particles.

Journal of molecular biology, 1985
Biosynthetic precursors of U2 small nuclear RNA have been identified in cultured human cells by hybrid-selection of pulse-labeled RNA with cloned U2 DNA. These precursor molecules are one to approximately 16 nucleotides longer than mature U2 RNA and contain 2,2,7-trimethylguanosine "caps".
E D, Wieben, J M, Nenninger, T, Pederson
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Functioning of the Drosophila integral U1/U2 protein Snf independent of U1 and U2 small nuclear ribonucleoprotein particles is revealed by snf(+) gene dose effects.

Proceedings of the National Academy of Sciences of the United States of America, 2000
Snf, encoded by sans fille, is the Drosophila homolog of mammalian U1A and U2B" and is an integral component of U1 and U2 small nuclear ribonucleoprotein particles (snRNPs). Surprisingly, changes in the level of this housekeeping protein can specifically affect autoregulatory activity of the RNA-binding protein Sex-lethal (Sxl) in an action that we ...
T W, Cline, D Z, Rudner, D A, Barbash, M, Bell, R, Vutien   +4 more
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Mechanics and functional consequences of nuclear deformations

Nature Reviews Molecular Cell Biology, 2022
Andrew Stephens, Sylvain Gabriele, Jan Lammerding   +2 more
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