Results 111 to 120 of about 333 (143)

U small nuclear ribonucleoprotein requirements for nematode cis- and trans-splicing in vitro

, 1991
In nematodes, a fraction of mRNAs acquires a common 22-nucleotide 5'-terminal spliced leader sequence via a trans-splicing reaction. The same premessenger RNAs which receive the spliced leader are also processed by conventional cis-splicing.
Maroney, P. A., Nilsen, T. W., Hannon, G. J.   +2 more
core  

Association of U2AF35, U2AF65 and U2 snRNP with constitutively and alternatively Spliced pre-mRNA

, 2016
In the early stages of splicing, introns are bounded by splice sites at the 5’ and 3’ ends. Candidate 5’ splice sites are bound by U1 snRNPs (small nuclear Ribonucleoprotein). The 3’ splice sites comprise three distinct sequence elements.
Li Chen (5749)
core   +1 more source

U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction

, 1991
U4 and U6 small nuclear RNAs reside in a single ribonucleoprotein particle, and both are required for pre-mRNA splicing. The U4/U6 and U5 small nuclear ribonucleoproteins join Ul and U2 on the pre-mRNA during spliceosome assembly.
Ren-jang Lin, Shyue-lee Yean
core  

U2 Small Nuclear Ribonucleoprotein Auxiliary Factor 35 kDa Subunit-Related Protein 2 [PDF]

, 2020
openaire   +1 more source

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Psoriasis and Raynaud's Phenomenon Associated with Autoantibodies to U1 and U2 Small Nuclear Ribonucleoproteins

New England Journal of Medicine, 1986
One of the hallmarks of systemic lupus erythematosus and related disorders is the production of antinuclear antibodies reactive with a variety of nuclear structures, including nucleic acids (DNA an...
W H, Reeves, D E, Fisher, R, Wisniewolski, A B, Gottlieb, N, Chiorazzi   +4 more
openaire   +2 more sources

U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

Cell, 1985
Two different experimental approaches have provided evidence that both U2 and U1 snRNPs function in pre-mRNA splicing. When the U2 snRNPs in a nuclear extract are selectively degraded using ribonuclease H and either of two deoxyoligonucleotides complementary to U2 RNA, splicing activity is abolished. Mixing an extract in which U2 has been degraded with
D L, Black, B, Chabot, J A, Steitz
openaire   +2 more sources

U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

Cell, 1986
The requirement for individual U RNAs in splicing and polyadenylation was investigated using oligonucleotide-directed cleavage of snRNAs in in vitro processing extracts. Cleavage of U1, U2, or U4 RNA inhibited splicing but not polyadenylation of short precursor RNAs.
S M, Berget, B L, Robberson
openaire   +2 more sources

Ribonucleoprotein particle assembly and modification of U2 small nuclear RNA containing 5-fluorouridine

Biochemistry, 1993
An in vitro assembly/modification system was used to study the effect of 5-fluorouridine (5-FU) incorporation on the biosynthesis of the U2 small nuclear ribonucleoprotein particle (U2 snRNP). Labeled U2 RNAs were transcribed in vitro with 5-fluoro-UTP either partially supplementing or completely replacing UTP during synthesis.
openaire   +2 more sources

Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for Pre-mRNA splicing in vitro

Cell, 1985
We have identified six distinct factors necessary for pre-mRNA splicing in vitro by selective inactivation and complementation studies, and by fractionation procedures. Splicing factor 1 (SF1) is sensitive to micrococcal nuclease, and appears to consist of at least U1 and U2 snRNPs, since splicing is inhibited when the 5' termini of U1 and U2 snRNAs ...
A R, Krainer, T, Maniatis
openaire   +2 more sources

Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome

Science, 1986
The splicing process, which removes intervening sequences from messenger RNA (mRNA) precursors is essential to gene expression in eukaryotic cells. This site-specific process requires precise sequence recognition at the boundaries of an intervening sequence, but the mechanism of this recognition is not understood.
P J, Grabowski, P A, Sharp
openaire   +2 more sources