Results 31 to 40 of about 57,169 (289)

Highly Efficient, Rapid and Co-CRISPR-Independent Genome Editing in Caenorhabditis elegans

open access: yesG3: Genes, Genomes, Genetics, 2017
We describe a rapid and highly efficient method to generate point mutations in Caenorhabditis elegans using direct injection of CRISPR-Cas9 ribonucleoproteins.
Harriet Prior   +3 more
doaj   +1 more source

Structural studies of protein–nucleic acid complexes: A brief overview of the selected techniques

open access: yesComputational and Structural Biotechnology Journal, 2023
Protein–nucleic acid complexes are involved in all vital processes, including replication, transcription, translation, regulation of gene expression and cell metabolism.
Kamil Szpotkowski   +2 more
doaj  

Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. [PDF]

open access: yes, 1994
To study the functions of heterogeneous nuclear ribonucleoproteins (hnRNPs), we have characterized nuclear polyadenylated RNA-binding (Nab) proteins from Saccharomyces cerevisiae.
Datar, KV   +4 more
core   +1 more source

Dysregulation of H/ACA ribonucleoprotein components in chronic lymphocytic leukemia [PDF]

open access: yes, 2017
Telomeres are protective repeats of TTAGGG sequences located at the end of human chromosomes. They are essential to maintain chromosomal integrity and genome stability.
Bezares, Raimundo F.   +6 more
core   +1 more source

Binding of RNA by the Nucleoproteins of Influenza Viruses A and B

open access: yesViruses, 2016
This paper describes a biochemical study for making complexes between the nucleoprotein of influenza viruses A and B (A/NP and B/NP) and small RNAs (polyUC RNAs from 5 to 24 nucleotides (nt)), starting from monomeric proteins.
Alice Labaronne   +5 more
doaj   +1 more source

A Streamlined Protocol for Wheat (Triticum aestivum) Protoplast Isolation and Transformation With CRISPR-Cas Ribonucleoprotein Complexes

open access: yesFrontiers in Plant Science, 2020
The genetic engineering method CRISPR has been touted as an efficient, inexpensive, easily used, and targeted genetic modification technology that is widely suggested as having the potential to solve many of the problems facing agriculture now and in the
Kali M. Brandt   +3 more
doaj   +1 more source

Synthetic gRNA/Cas9 Ribonucleoprotein Inhibits HIV Reactivation and Replication

open access: yesViruses, 2022
The current antiretroviral therapy (ART) for human immunodeficiency virus (HIV) can halt viral replication but cannot eradicate HIV infection because proviral DNA integrated into the host genome remains genetically silent in reservoir cells and is ...
Sushant Khanal   +14 more
doaj   +1 more source

The synthesis of hemoglobin in a cell-free system [PDF]

open access: yes, 1958
The microsomal particles (1) have been implicated as the major sites of protein synthesis within the cells of a variety of tissues. The studies of Borsook, Zamecnik, Hultin, and others, with intact animals and various types of whole cell systems (see ...
Allen, Esther   +2 more
core   +2 more sources

A candidate protective factor in amyotrophic lateral sclerosis: heterogenous nuclear ribonucleoprotein G

open access: yesNeural Regeneration Research, 2023
Heterogenous nuclear ribonucleoprotein G is down-regulated in the spinal cord of the Tg(SOD1*G93A)1Gur (TG) amyotrophic lateral sclerosis mouse model.
Fang Yang   +4 more
doaj   +1 more source

Cell division promotes efficient retrotransposition in a stable L1 reporter cell line [PDF]

open access: yes, 2013
Background: Long interspersed element type one (L1) actively modifies the human genome by inserting new copies of itself. This process, termed retrotransposition, requires the formation of an L1 ribonucleoprotein (RNP) complex, which must enter the ...
An, Wenfeng   +5 more
core   +2 more sources

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