Results 21 to 30 of about 95,245 (266)
Genome Editing in Bacteria: CRISPR-Cas and Beyond
Microorganisms, 2021 Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids. The discovery and applications of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas based technologies have ...
Ruben D. Arroyo-Olarte +2 more
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Frontiers in Plant Science, 2020 The genetic engineering method CRISPR has been touted as an efficient, inexpensive, easily used, and targeted genetic modification technology that is widely suggested as having the potential to solve many of the problems facing agriculture now and in the
Kali M. Brandt +3 more
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Frontiers in Microbiology, 2022 The mini-genome reporter assay is a key tool for conducting RNA virus research. However, procedural complications and the lack of adequate literature pose a major challenge in developing these assay systems.
Nandita Kedia +2 more
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Binding of RNA by the Nucleoproteins of Influenza Viruses A and B
Viruses, 2016 This paper describes a biochemical study for making complexes between the nucleoprotein of influenza viruses A and B (A/NP and B/NP) and small RNAs (polyUC RNAs from 5 to 24 nucleotides (nt)), starting from monomeric proteins.
Alice Labaronne +5 more
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A novel experimental approach for the selective isolation and characterization of human RNase MRP
RNA Biology, 2022 RNase MRP is a ribonucleoprotein complex involved in the endoribonucleolytic cleavage of different RNAs. Mutations in the RNA component of the RNP are the cause of cartilage hair hypoplasia.
Merel Derksen +5 more
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Synthetic gRNA/Cas9 Ribonucleoprotein Inhibits HIV Reactivation and Replication
Viruses, 2022 The current antiretroviral therapy (ART) for human immunodeficiency virus (HIV) can halt viral replication but cannot eradicate HIV infection because proviral DNA integrated into the host genome remains genetically silent in reservoir cells and is ...
Sushant Khanal +14 more
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HIV-1 Recruits UPF1 but Excludes UPF2 to Promote Nucleocytoplasmic Export of the Genomic RNA
Biomolecules, 2015 Unspliced, genomic HIV-1 RNA (vRNA) is a component of several ribonucleoprotein complexes (RNP) during the viral replication cycle. In earlier work, we demonstrated that the host upframeshift protein 1 (UPF1), a key factor in nonsense-mediated mRNA decay
Lara Ajamian +8 more
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Highly Efficient, Rapid and Co-CRISPR-Independent Genome Editing in Caenorhabditis elegans
G3: Genes, Genomes, Genetics, 2017 We describe a rapid and highly efficient method to generate point mutations in Caenorhabditis elegans using direct injection of CRISPR-Cas9 ribonucleoproteins.
Harriet Prior +3 more
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Neural Regeneration Research, 2023 Heterogenous nuclear ribonucleoprotein G is down-regulated in the spinal cord of the Tg(SOD1*G93A)1Gur (TG) amyotrophic lateral sclerosis mouse model.
Fang Yang +4 more
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Dissection of affinity captured LINE-1 macromolecular complexes
eLife, 2018 Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs).
Martin S Taylor +16 more
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