Results 31 to 40 of about 95,245 (266)

Large-scale remodeling of a repressed exon ribonucleoprotein to an exon definition complex active for splicing

eLife, 2016
Polypyrimidine-tract binding protein PTBP1 can repress splicing during the exon definition phase of spliceosome assembly, but the assembly steps leading to an exon definition complex (EDC) and how PTBP1 might modulate them are not clear.
Somsakul Pop Wongpalee, Ajay Vashisht, Shalini Sharma, Darryl Chui, James A Wohlschlegel, Douglas L Black   +5 more
doaj   +1 more source

Enhanced Ribonucleoprotein Immunoprecipitation (RIP) Technique for the Identification of mRNA Species in Ribonucleoprotein Complexes

Bio-Protocol, 2022
RNA binding proteins (RBPs) are critical regulators of cellular phenotypes, and dysregulated RBP expression is implicated in various diseases including cancer.
Saja Fakhraldeen, Scott Berry, David Beebe, Caroline Alexander   +3 more
doaj   +1 more source

MAFG-AS1 is a prognostic biomarker and facilitates prostate cancer progression

Frontiers in Oncology, 2022
Long Noncoding RNAs (LncRNAs) have recently been identified as key regulator in tumor progression. The LncRNA MAFG-AS1 has been reported to facilitate the progression of multiple cancers, however, its role in prostate cancer is still unknown.
Peizhang Li, Yuanping Shi, Miaomiao Guo, Miaomiao Guo, Huan Xu, Ming Zhan, Zhong Wang, Yanbo Chen   +7 more
doaj   +1 more source

Possible role of human ribonuclease dicer in the regulation of R loops

FEBS Open Bio
R loops are three‐stranded nucleic acid structures that form naturally in cells under various conditions, mainly as intermediates during replication or as by‐products during transcription. R loops are involved in the regulation of many important cellular
Klaudia Wojcik, Paulina Krzeminska, Anna Kurzynska‐Kokorniak   +2 more
doaj   +1 more source

Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations

Communications Biology, 2021
Fujita et al. investigate the use of oligoribonucleotides, proteins, and ribonucleoprotein complexes as sequence-specific blockers of DNA extension by DNA polymerases.
Toshitsugu Fujita, Shoko Nagata, Hodaka Fujii   +2 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

The human Dicer helicase domain is capable of ATP hydrolysis and single-stranded nucleic acid binding

BMC Biology
Background Vertebrates have one Dicer ortholog that generates both microRNAs (miRNAs) and small interfering RNAs (siRNAs), in contrast to the multiple Dicer-like proteins found in flies and plants.
Kinga Ciechanowska, Agnieszka Szczepanska, Kamil Szpotkowski, Klaudia Wojcik, Anna Urbanowicz, Anna Kurzynska-Kokorniak   +5 more
doaj   +1 more source

Analysis of the extranucleolar ribonucleoprotein particles of Cycas revoluta Thunb. (Cycadaceae) and Ceratozamia mexicana Brongn. (Zamiaceae)

Botan‪ical Sciences, 2022
Background: Nuclear ribonucleoprotein particles play a key role in RNA processing and in the gene expression pathway. Interchromatin granules (GICs) involved in the metabolism of pre-messenger RNA (pre-mRNA) were described in Allium cepa and ...
Lourdes-Teresa Agredano-Moreno, María de Lourdes Segura-Valdez, Jaime Jiménez-Ramírez, Luis-Felipe Jiménez-García   +3 more
doaj   +1 more source

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Efficient multi-allelic genome editing via CRISPR–Cas9 ribonucleoprotein-based delivery to Brassica napus mesophyll protoplasts

Frontiers in Plant Science
Canola (Brassica napus L.) is a valuable oilseed crop worldwide. However, trait improvement by breeding has been limited by its low genetic diversity and polyploid genetics.
Sareena Sahab, Fatima Runa, Mahilini Ponnampalam, Pippa T. Kay, Elizabeth Jaya, Katerina Viduka, Stephen Panter, Josquin Tibbits, Josquin Tibbits, Matthew J. Hayden, Matthew J. Hayden   +10 more
doaj   +1 more source