Results 111 to 120 of about 30,834 (284)

Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans [PDF]

, 2018
Background: Advances in automated image-based microscopy platforms coupled with high-throughput liquid workflows have facilitated the design of large-scale screens utilising multicellular model organisms such as Caenorhabditis elegans to identify genetic
Artal Sanz, Marta, Askjaer, Peter, Baumeister, Ralf, Erinjeri, Annmary Paul, González Hernández, Sara, Hernando Rodríguez, Blanca, Millar, Val, Muñoz, Manuel J., Olmedo López, María, Rodríguez Palero, María Jesús, Schulze, Bettina   +10 more
core   +1 more source

Extracellular Vesicle‐Based mRNA Therapeutics and Vaccines

Exploration, EarlyView.
This review provides a comprehensive analysis of recent advancements in extracellular vesicle (EV)‐based messenger RNA (mRNA) applications for therapeutics and vaccines. It systematically outlines strategies for mRNA encapsulation within EVs and elucidates the challenges associated with this technology, offering insights into future research directions
Qi Li, Haonan Xing, Abid Naeem, Kaiyue Zhang, Aiping Zheng, Yuanyu Huang, Mei Lu   +6 more
wiley   +1 more source

A creature with a hundred waggly tails: intrinsically disordered proteins in the ribosome [PDF]

, 2013
This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Dunker, A. Keith, Kurgan, Lukasz, Mizianty, Marcin J., Oldfield, Christopher J., Peng, Zhenling, Uversky, Vladmir N., Xue, Bin   +6 more
core   +2 more sources

Emerging Target Discovery Strategies Drive the Decoding of Therapeutic Power of Natural Products and Further Drug Development: A Case Study of Celastrol

Exploration, EarlyView.
Using celastrol as a case study, this review summarizes various target discovery strategies for natural products, including chemical proteomics, protein microarray, degradation‐based protein profiling, proteome‐wide label‐free approaches, network pharmacology, target‐based drug screening, and indirect strategies.
Yanbei Tu, Guiyu Dai, Yanyan Chen, Lihua Tan, Hanqing Liu, Meiwan Chen   +5 more
wiley   +1 more source

Signal Recognition Particle 54 kD Protein (SRP54) from the Marine Sponge Geodia cydonium

Food Technology and Biotechnology, 2002
In the systematic search for phylogenetically conserved proteins in the simplest and most ancient extant metazoan phylum – Porifera, we have identified and analyzed a cDNA encoding the signal recognition particle 54 kD protein (SRP54) from the marine ...
Sonja Durajlija-Žinić, Helena Četković, Werner E. G. Müller, Vera Gamulin   +3 more
doaj  

Hermes (Rbpms) is a Critical Component of RNP Complexes that Sequester Germline RNAs during Oogenesis

Journal of Developmental Biology, 2016
The germ cell lineage in Xenopus is specified by the inheritance of germ plasm that assembles within the mitochondrial cloud or Balbiani body in stage I oocytes. Specific RNAs, such as nanos1, localize to the germ plasm. nanos1 has the essential germline
Tristan Aguero, Yi Zhou, Malgorzata Kloc, Patrick Chang, Evelyn Houliston, Mary Lou King   +5 more
doaj   +1 more source

A promising frontier of circulating messenger RNA in liquid biopsy: From mechanisms to clinical applications

International Journal of Cancer, EarlyView.
Abstract Liquid biopsy can revolutionize cancer patient management as repeated sampling allows real‐time monitoring of disease progression and response to treatment. The circulating transcriptome represents a rich source of potential cancer biomarkers, including coding and non‐coding RNAs.
Mengqian Li, Yijin Yu, Guangyue Liu, Shunhong Zhang, Chenchen Luo, Saidi Hu, Siran Wan, Linyong Zhao   +7 more
wiley   +1 more source

Single-particle mass analysis of intact ribosomes by mass photometry and Orbitrap-based charge detection mass spectrometry

iScience, 2021
Summary: Standard methods for mass analysis measure ensembles of thousand to millions of molecules. This approach enables analysis of monodisperse recombinant proteins, whereas some heterogeneous protein assemblies pose a significant challenge, whereby ...
Szu-Hsueh Lai, Sem Tamara, Albert J.R. Heck   +2 more
doaj  

New insights into applications of base editor in hereditary disorders

Interdisciplinary Medicine, EarlyView.
Abstract Hereditary disorders are a group of diseases caused by genetic mutations or chromosomal variations. Although the incidence of each genetic disorder is relatively low, patients affected by the disease generally experience a range of severe symptoms, including blindness, disability, and even premature death. In addition, the available treatments
Maoping Cai, Linhui Zhang, Liqing Li, Yue Liu, Canbin Lv, Yan Shi, Jianyuan Zhao, Dingwei Ye, Yuanyuan Qu   +8 more
wiley   +1 more source

Potent programmable antiviral against dengue virus in primary human cells by Cas13b RNP with short spacer and delivery by VLP

Molecular Therapy: Methods & Clinical Development, 2021
With sequencing as a standard frontline protocol to identify emerging viruses such Zika virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), direct utilization of sequence data to program antivirals against the viruses could accelerate
Ekapot Singsuksawat, Suppachoke Onnome, Pratsaneeyaporn Posiri, Amporn Suphatrakul, Nittaya Srisuk, Rapirat Nantachokchawapan, Hansa Praneechit, Chutimon Sae-kow, Pala Chidpratum, Khanit Sa-ngiamsuntorn, Suradej Hongeng, Panisadee Avirutnan, Thaneeya Duangchinda, Bunpote Siridechadilok   +13 more
doaj