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Structure and Function of Nuclear and Cytoplasmic Ribonucleoprotein Particles [PDF]
NUCLEAR RIBONUCLEOPROTEIN PARTICLES . Evidence for, and Isolation of, hnRNP Particles . General Structural Features of hnRNP Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. Proteins of hnRNP Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...... . . . . . . .
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Ribonucleoprotein particles: advances and challenges in computational methods
Current Opinion in Structural Biology, 2018RNA-binding proteins (RBPs) interact with RNA to form Ribonucleoprotein Particles (RNPs). The interaction between RBPs and their RNA partners are traditionally thought to be mediated by highly conserved RNA-binding domains (RBDs). Recently, high-throughput studies led to the discovery of hundreds of novel proteins and domains, of which many do not ...
Shlomi Dvir+2 more
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Messenger Ribonucleoprotein Particles
1982Messenger RNA, from the time of its synthesis in the nucleus through its translation and degradation, does not exist as free RNA in animal cells but rather always appear to be complexed with proteins. Free cytoplasmic ribonucleoprotein (RNP) particles containing non-ribosomal RNA were discovered by Spirin and coworkers (1) in 1964 and were called ...
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Phosphorus imaging of the 7-S ribonucleoprotein particle
Journal of Ultrastructure and Molecular Structure Research, 1988In this study an unprecedented demonstration of the detection sensitivity of electron spectroscopic imaging (ESI) is reported. This microanalytical technique is capable of forming elemental maps of a specimen with high sensitivity and resolution by forming images with electrons that have lost particular amounts of energy due to interactions with the ...
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Analysis of the molecular composition of Ro ribonucleoprotein particles
Molecular Biology Reports, 1990Gustav Fabini, Saskia A. Rutjes, Christof Zimmermann, Ger J. M. Pruijn and GuE nter Steiner Institute of Biochemistry, University of Vienna, Austria; Division of Rheumatology, University Hospital of Vienna, Austria; Department of Biochemistry, University of Nijmegen, the Netherlands; 2nd Department of Medicine, Lainz Hospital, Vienna, Austria; Ludwig ...
Ger J. M. Pruijn+2 more
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Extraction and Quantitative Precipitation of Nuclear Ribonucleoprotein Particles
Preparative Biochemistry, 1986Ribonucleoprotein particles (RNPs) were extracted from monkey cell nuclei in media of low ionic strength. The rapidly labeled RNPs were comparable in terms of size, protein patterns and protein content to those extracted by sonication. The overnight labeled RNPs were homogenous (sedimenting at 60-65S and containing RNA of 30-31S), and appear to be a ...
Perpetua M. Muganda-ojiaku+1 more
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ROD-SHAPED RIBONUCLEOPROTEIN PARTICLES FROM SAPROSPIRA
Canadian Journal of Microbiology, 1964not ...
David L. Correll, Ralph A. Lewin
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cDNA library generation from ribonucleoprotein particles
Nature Protocols, 2011Most, if not all, known noncoding RNAs (ncRNAs) are associated with RNA binding proteins, thus forming ribonucleoprotein particles or RNPs. Here we describe a protocol for the generation of a specialized cDNA library from RNPs, thereby increasing the proportion of functional ncRNA species in the library.
Alexander Hüttenhofer+1 more
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Auto-Antigens of Nuclear Ribonucleoprotein Particles
1985Abstract Sm and RNP antigens are present on duck erythroblast nuclear ribonucleoproteins, and they are identical to those of calf thymus and human tumoral cells Hep 2. Epitopes of these antigens are present on the proteins obtained after phenol extraction of the nuclear RNP from these different origins.
Homberg Jc+3 more
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RNA-Protein Interactions in Ribonucleoprotein Particles [PDF]
Genetic information stored as the nucleotide sequence of DNA is transcribed into RNA during gene expression. RNA not only carries genetic information from DNA to protein as messenger-RNA (mRNA) but also forms an integral part of the ribosome (Noller 1991) pre-mRNA splicing machinery (Sharp 1987; Steitz et al. 1988; Luhrmann et al.
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