Results 101 to 110 of about 77,265 (266)

Plant organelle C‐to‐U RNA editing factors can operate successfully in yeast (Saccharomyces cerevisiae) as an easily amenable eukaryotic system for their functional analysis

The FEBS Journal, EarlyView.
Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan, Annika Hanraths, Jingchan Xie, Mareike Schallenberg‐Rüdinger, Volker Knoop   +4 more
wiley   +1 more source

Structure-guided design of anti-cancer ribonucleotide reductase inhibitors

Journal of Enzyme Inhibition and Medicinal Chemistry, 2019
Ribonucleotide reductase (RR) catalyses the rate-limiting step of dNTP synthesis, establishing it as an important cancer target. While RR is traditionally inhibited by nucleoside-based antimetabolites, we recently discovered a naphthyl salicyl acyl ...
Tessianna A. Misko, Yi-Ting Liu, Michael E. Harris, Nancy L. Oleinick, John Pink, Hsueh-Yun Lee, Chris G. Dealwis   +6 more
doaj   +1 more source

Interaction Between The MEC1-Dependent DNA Synthesis Checkpoint And G1 Cyclin Function In Saccharomyces Cerevisiae [PDF]

, 1999
The completion of DNA synthesis in yeast is monitored by a checkpoint that requires MEC1 and RAD53. Here we show that deletion of the Saccharomyces cerevisiae G1 cyclins CLN1 and CLN2 suppressed the essential requirement for MEC1 function.
Cross, F. R., Vallen, Elizabeth Ann
core   +1 more source

Bacillus subtilis RNase HII Is Inefficient at Processing Guanosine Monophosphate and Damaged Ribonucleotides

Molecular Microbiology, EarlyView.
Bacillus subtilis RNase HII corrects ribonucleotide errors and mismatched ribonucleotides, but not damaged ribonucleotides. Mismatched ribonucleotides would also serve as substrates for mismatch repair while damaged ribonucleotides would be addressed through base excision repair.
Julianna R. Cresti, Lyle A. Simmons
wiley   +1 more source

Boosting Reversible Photocontrol of a Photoxenase by an Engineered Conformational Shift

Angewandte Chemie, Volume 138, Issue 4, 22 January 2026.
Photocontrol of enzymes with photoswitchable unnatural amino acids hitherto yielded only limited light‐regulation factors (LRFs). Here we boosted the LRF of an imidazole glycerol phosphate synthase variant from ∼15 to ∼100 in a semi‐rational design approach and unraveled the mechanistic fundamentals of this effect via biophysical and computational ...
Sabrina Mandl, Janet Sánchez, Miquel Estévez, Astrid Bruckmann, Caroline Hiefinger, Sílvia Osuna, Andrea Hupfeld   +6 more
wiley   +2 more sources

KRP3 Stability Controls Rice Plant Architecture and Productivity via MPK3‐Mediated Phosphorylation

Plant Biotechnology Journal, EarlyView.
ABSTRACT Yield is a critical agronomic trait in cereal crops, shaped by factors like tiller and seed number, and seed weight. Understanding the factors governing these traits will help in improving the yield of plants. In this study, we identified an orphan gene, KRP3, belonging to cereal crops as a key regulator of rice plant architecture.
Gopal Banerjee, Sarvesh Jonwal, Balakrishnan Rengasamy, Uttam Pal, Dhanraj Singh, Mohit Mohit, Alok Krishna Sinha   +6 more
wiley   +1 more source

Translation‐Promoting Effects of RNA Template Overhangs in the Absence of Ribosomes

Angewandte Chemie, Volume 138, Issue 4, 22 January 2026.
Ribosome‐free translation of an RNA sequence into a peptide sequence can be controlled by the folding of the template strand. Overhang sequences forming a triplex structure with the templating region and the peptidoyl RNA in pH‐dependent manner increase yields up to 40‐fold through entropic effects and mixed anhydride formation, allowing for controlled,
Nikolaos Giannakopoulos, Martin Rentschler, Clemens Richert   +2 more
wiley   +2 more sources

Substrate Properties of Fluorescent Ribonucleotides in the Terminal Transferase-Catalyzed Labeling of DNA Sequencing Primers

BioTechniques, 1996
Terminal deoxynucleotidyltransferase (terminal transferase, E.C. 2.7.7.31) has been used to add a single fluorescent ribonucleotide to the 3′ terminus of DNA sequencing primers, thereby creating primers suitable for automated DNA sequence analysis.
Gabor L. Igloi
doaj   +1 more source

ZmTH1 Is Vital for Healthy Plant Growth and Promotes Cold/Drought Tolerance by Regulating Thiamin Diphosphate‐Dependent Metabolisms in Maize

Plant Biotechnology Journal, EarlyView.
ABSTRACT Vitamin B1 (VB1) plays a crucial role in sustaining plant health and enabling adaptive responses to environmental stress. The complex maize genome implies a sophisticated VB1 synthesis pathway, with the mechanisms by which VB1 benefits plants remaining elusive.
Tengfei Zhang, Jie Zang, Boming Yang, Qiuxia Wang, Jijun Yan, Peiyong Xin, Jinfang Chu, Huabang Chen, Zhaogui Zhang   +8 more
wiley   +1 more source

Universal Restriction Site-Free Cloning Method Using Chimeric Primers

BioTechniques, 2002
A universal restriction site-free cloning method has been developed to precisely insert a DNA fragment into a vector at any desired location without altering any nucleotide(s) in either the DNA fragment or the vector.
Guo Jun Chen, Nahong Qiu, Malcolm G.P. Page   +2 more
doaj   +1 more source