Results 51 to 60 of about 48,825 (277)

Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase

Chemical Science, 2018
Specific and sensitive detection of DNA MTase activity can be achieved by a single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification approach.
Li-juan Wang, Xiao Han, Chen-chen Li, Chun-yang Zhang   +3 more
semanticscholar   +1 more source

Nucleotide pools dictate the identity and frequency of ribonucleotide incorporation in mitochondrial DNA

PLoS Genetics, 2017
Previous work has demonstrated the presence of ribonucleotides in human mitochondrial DNA (mtDNA) and in the present study we use a genome-wide approach to precisely map the location of these.
A. Berglund, Clara Navarrete, M. Engqvist, E. Hoberg, Z. Szilágyi, Robert W. Taylor, C. Gustafsson, M. Falkenberg, A. Clausen   +8 more
semanticscholar   +1 more source

Aberrant ribonucleotide incorporation and multiple deletions in mitochondrial DNA of the murine MPV17 disease model

Nucleic Acids Research, 2017
All DNA polymerases misincorporate ribonucleotides despite their preference for deoxyribonucleotides, and analysis of cultured cells indicates that mammalian mitochondrial DNA (mtDNA) tolerates such replication errors.
C. Moss, I. Dalla Rosa, L. Hunt, T. Yasukawa, Robert S. Young, A. W. Jones, Kaalak Reddy, R. Desai, S. Virtue, G. Elgar, P. Voshol, Martin S. Taylor, I. Holt, Martin A. M. Reijns, A. Spinazzola   +14 more
semanticscholar   +1 more source

Structural accommodation of ribonucleotide incorporation by the DNA repair enzyme polymerase Mu

Nucleic Acids Research, 2017
While most DNA polymerases discriminate against ribonucleotide triphosphate (rNTP) incorporation very effectively, the Family X member DNA polymerase μ (Pol μ) incorporates rNTPs almost as efficiently as deoxyribonucleotides.
A. F. Moon, J. Pryor, D. Ramsden, T. Kunkel, K. Bebenek, L. Pedersen   +5 more
semanticscholar   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Manipulating the Bacterial Cell Cycle and Cell Size by Titrating the Expression of Ribonucleotide Reductase

mBio, 2017
Understanding how bacteria coordinate growth with cell cycle events to maintain cell size homeostasis remains a grand challenge in biology. The period of chromosome replication (C period) is a key stage in the bacterial cell cycle. However, the mechanism
Manlu Zhu, Xiongfeng Dai, W. Guo, Zengxiang Ge, Ming-Tzong Yang, Haikuan Wang, Yi-Ping Wang   +6 more
semanticscholar   +1 more source

Elucidating prognostic significance of purine metabolism in colorectal cancer through integrating data from transcriptomic, immunohistochemical, and single‐cell RNA sequencing analysis

Molecular Oncology, EarlyView.
Low expression of five purine metabolism‐related genes (ADSL, APRT, ADCY3, NME3, NME6) was correlated with poor survival in colorectal cancer. Immunohistochemistry analysis showed that low NME3 (early stage) and low ADSL/NME6 (late stage) levels were associated with high risk.
Sungyeon Kim, Myunghee Kang, Soyeon Jeong, Jisup Kim, Kyoung Oh Kim, Won‐Suk Lee, Jeong‐Heum Baek, Jung Ho Kim, Seungyoon Nam   +8 more
wiley   +1 more source

A nucleotide-sensing oligomerization mechanism that controls NrdR-dependent transcription of ribonucleotide reductases

Nature Communications, 2022
Ribonucleotide reductase (RNR) is an essential enzyme that catalyzes the synthesis of DNA building blocks. Here, the authors present the cryo-EM structure and mechanism of action of NrdR, the RNR-specific repressor, that controls transcription of RNR ...
Inna Rozman Grinberg, Markel Martínez-Carranza, Ornella Bimai, Ghada Nouaïria, Saher Shahid, Daniel Lundin, Derek T. Logan, Britt-Marie Sjöberg, Pål Stenmark   +8 more
doaj   +1 more source

P53 suppresses ribonucleotide reductase via inhibiting mTORC1

OncoTarget, 2017
Balanced deoxyribonucleotides pools are essential for cell survival and genome stability. Ribonucleotide reductase is the rate-limiting enzyme for the production of deoxyribonucleotides. We report here that p53 suppresses ribonucleotide reductase subunit
Zhengfu He, Xing Hu, Weijing Liu, A. Dorrance, R. Garzon, P. Houghton, Changxian Shen   +6 more
semanticscholar   +1 more source