Results 71 to 80 of about 48,825 (277)

Topoisomerase I Alone Is Sufficient to Produce Short DNA Deletions and Can Also Reverse Nicks at Ribonucleotide Sites*

Journal of Biological Chemistry, 2015
Background: DNA deletions at short repeat sites containing ribonucleotides are Top1-dependent. Results: Top1 generates DNA deletions at short repeat sites in vitro and also reverses nicks at ribonucleotide sites.
Shar-yin N. Huang, Sanchari Ghosh, Y. Pommier   +2 more
semanticscholar   +1 more source

The fate of thiopurine metabolites after switching to low‐dose thiopurine with allopurinol or thioguanine in IBD patients: A retrospective analysis

British Journal of Clinical Pharmacology, EarlyView.
Aims Shunting (hypermethylating) thiopurine metabolism, characterized by excessive 6‐MMPR production and (sub)therapeutic 6‐TGN levels, poses a significant challenge in the treatment of inflammatory bowel disease (IBD). This study evaluates the metabolic outcomes of switching to low‐dose thiopurine with allopurinol (LDTA) or thioguanine (TG) in IBD ...
Ahmed B. Bayoumy, Hedwig M. A. D'Agnolo, Nanne K. H. de Boer, Chris J. J. Mulder, Luc J. J. Derijks   +4 more
wiley   +1 more source

A New Type of YumC-Like Ferredoxin (Flavodoxin) Reductase Is Involved in Ribonucleotide Reduction

mBio, 2015
The trxB2 gene, which is annotated as a thioredoxin reductase, was found to be essential for growth of Lactococcus lactis in the presence of oxygen. The corresponding protein (TrxB2) showed a high similarity with Bacillus subtilis YumC (E value = 4.0E−88)
Jun Chen, Jing Shen, Christian Solem, Peter Ruhdal Jensen   +3 more
doaj   +1 more source

Diversity in Overall Activity Regulation of Ribonucleotide Reductase*

Journal of Biological Chemistry, 2015
Background: Ribonucleotide reductase (RNR) makes DNA building blocks. Results: Binding of three dATP molecules to the Pseudomonas aeruginosa class I RNR α subunit inactivates the enzyme by inducing an inert α4 complex.
V. Jonna, Mikael Crona, R. Rofougaran, D. Lundin, S. Johansson, K. Brännström, B. Sjöberg, A. Hofer   +7 more
semanticscholar   +1 more source

The CRISPR-Cas9 crATIC HeLa transcriptome: Characterization of a novel cellular model of ATIC deficiency and ZMP accumulation

Molecular Genetics and Metabolism Reports, 2020
In de novo purine biosynthesis (DNPS), 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase (EC 2.1.2.3)/inosine monophosphate cyclohydrolase (EC 3.5.4.10) (ATIC) catalyzes the last two reactions of the pathway: conversion of 5-aminoimidazole ...
Randall C. Mazzarino, Veronika Baresova, Marie Zikánová, Nathan Duval, Terry G. Wilkinson, II, David Patterson, Guido N. Vacano   +6 more
doaj  

Impact of Ribonucleotide Backbone on Translesion Synthesis and Repair of 7,8-Dihydro-8-oxoguanine*

Journal of Biological Chemistry, 2016
Numerous ribonucleotides are incorporated into the genome during DNA replication. Oxidized ribonucleotides can also be erroneously incorporated into DNA.
A. Sassa, Melike Çağlayan, Y. Rodriguez, W. Beard, Samuel H. Wilson, T. Nohmi, M. Honma, M. Yasui   +7 more
semanticscholar   +1 more source

Milligram‐Scale Enzymatic Synthesis of Base‐Modified DNA through Primer Extension: Polymerase Synthesis and NMR Structures of DNA Containing Phenyl‐Substituted Nucleobases

ChemistryEurope, EarlyView.
Larger‐scale enzymatic synthesis of base‐modified DNA is developed using primer extension with KOD(exo‐) DNA polymerase allowing preparation of milligram amounts of the modified DNA sufficient for NMR structural analysis. Milligram (≈200 nmol) scale enzymatic synthesis of base‐modified DNA is developed based on primer extension using easily accessible ...
Veronika Raindlová, Klára Viktorinová, Michal Tichý, Lucie Bednárová, Pavel Srb, Miguel Riopedre‐Fernandez, Michal Hocek   +6 more
wiley   +1 more source

Ribonucleotide reductases: essential enzymes for bacterial life

Frontiers in Cellular and Infection Microbiology, 2014
Ribonucleotide reductase (RNR) is a key enzyme that mediates the synthesis of deoxyribonucleotides, the DNA precursors, for DNA synthesis in every living cell.
E. Torrents
semanticscholar   +1 more source

NIR II‐Guided Photoactivatable Silencing Polyplex Boosts Cancer Immunotherapy

Exploration, EarlyView.
Due to limitation of penetration therapy with first and second‐generation photodynamic therapy (PDT) photosensitizers, an NIR II‐guided photoactivatable complex was proposed via the self‐assembly of the photosensitive polymer PTSQ and the electrostatic adsorption of siPD‐L1 to enhance cancer immunotherapy.
Yuquan Zhang, Jie Wang, Tian Zhang, Dongsheng Tang, Haiyin Yang, Shuai Guo, Yuchuan Fan, Caixia Sun, Haihua Xiao, Yuanyu Huang, Yuhua Weng   +10 more
wiley   +1 more source

Role of RNase H1 in DNA repair: removal of single ribonucleotide misincorporated into DNA in collaboration with RNase H2

Scientific Reports, 2015
Several RNases H1 cleave the RNA-DNA junction of Okazaki fragment-like RNA-DNA/DNA substrate. This activity, termed 3’-junction ribonuclease (3’-JRNase) activity, is different from the 5’-JRNase activity of RNase H2 that cleaves the 5’-side of the ...
Elias A Tannous, E. Kanaya, S. Kanaya
semanticscholar   +1 more source