Results 1 to 10 of about 9,310 (137)
Dehydration of Ribonucleotides Catalyzed by Ribonucleotide Reductase: The Role of the Enzyme [PDF]
Biophysical Journal, 2006 This article focuses on the second step of the catalytic mechanism for the reduction of ribonucleotides catalyzed by the enzyme Ribonucleotide Reductase (RNR). This step corresponds to the protonation/elimination of the substrate's C-2' hydroxyl group.
Leif A. Eriksson +3 more
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RIBONUCLEOTIDE REDUCTASES [PDF]
Annual Review of Biochemistry, 1998 Ribonucleotide reductases provide the building blocks for DNA replication in all living cells. Three different classes of enzymes use protein free radicals to activate the substrate. Aerobic class I enzymes generate a tyrosyl radical with an iron-oxygen center and dioxygen, class II enzymes employ adenosylcobalamin, and the anaerobic class III enzymes
A, Jordan, P, Reichard
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The periodic table of ribonucleotide reductases [PDF]
Journal of Biological Chemistry, 2021 In most organisms, transition metal ions are necessary cofactors of ribonucleotide reductase (RNR), the enzyme responsible for biosynthesis of the 2'-deoxynucleotide building blocks of DNA. The metal ion generates an oxidant for an active site cysteine (Cys), yielding a thiyl radical that is necessary for initiation of catalysis in all RNRs.
Terry B. Ruskoski, Amie K. Boal
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Ribonucleotides in bacterial DNA [PDF]
Critical Reviews in Biochemistry and Molecular Biology, 2014 In all living cells, DNA is the storage medium for genetic information. Being quite stable, DNA is well-suited for its role in storage and propagation of information, but RNA is also covalently included in DNA through various mechanisms. Recent studies also demonstrate useful aspects of including ribonucleotides in the genome during repair.
Justin R. Randall +3 more
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Identification of a novel type of spacer element required for imprinting in fission yeast [PDF]
, 2010 Asymmetrical segregation of differentiated sister chromatids is thought to be important for cellular differentiation in higher eukaryotes. Similarly, in fission yeast, cellular differentiation involves the asymmetrical segregation of a chromosomal ...
A Kaykov +47 more
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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis [PDF]
Journal of Visualized Experiments, 2018 The presence of ribonucleotides in nuclear DNA has been shown to be a source of genomic instability. The extent of ribonucleotide incorporation can be assessed by alkaline hydrolysis and gel electrophoresis as RNA is highly susceptible to hydrolysis in alkaline conditions.
Thomas A. Kunkel +2 more
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Impact of template backbone heterogeneity on RNA polymerase II transcription. [PDF]
, 2014 Variations in the sugar component (ribose or deoxyribose) and the nature of the phosphodiester linkage (3'-5' or 2'-5' orientation) have been a challenge for genetic information transfer from the very beginning of evolution.
Chong, Jenny +5 more
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Ribonucleotides in mitochondrial
FEBS Letters, 2019 The incorporation of ribonucleotides (rNMPs) into DNA during genome replication has gained substantial attention in recent years and has been shown to be a significant source of genomic instability. Studies in yeast and mammals have shown that the two genomes, the nuclear DNA (nDNA) and the mitochondrial DNA (mtDNA), differ with regard to their rNMP ...
Wanrooij, Paulina H., Chabes, Andrei
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Parallel analysis of ribonucleotide-dependent deletions produced by yeast Top1 in vitro and in vivo [PDF]
, 2016 Ribonucleotides are the most abundant non-canonical component of yeast genomic DNA and their persistence is associated with a distinctive mutation signature characterized by deletion of a single repeat unit from a short tandem repeat.
Burgers, Peter M +5 more
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Cold Spring Harbor Perspectives in Biology, 2010 It has normally been assumed that ribonucleotides arose on the early Earth through a process in which ribose, the nucleobases, and phosphate became conjoined. However, under plausible prebiotic conditions, condensation of nucleobases with ribose to give beta-ribonucleosides is fraught with difficulties.
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