Results 21 to 30 of about 17,369 (190)
Global Run-On sequencing to measure nascent transcription in C. elegans
STAR Protocols, 2021 Summary: Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level.
Piergiuseppe Quarato, Germano Cecere
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Preparation of DNA interstrand cross-link repair intermediates induced by abasic sites
MethodsX, 2022 DNA interstrand cross-links (ICLs) are extremely deleterious DNA lesions, which can block different DNA transactions. A major step in ICL repair involves strand cleavage activities flanking the cross-linking site, also known as unhooking.
Jin Tang, Linlin Zhao
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Ribonucleotide and R-Loop Damage in Plastid DNA and Mitochondrial DNA during Maize Development
Plants, 2023 Although the temporary presence of ribonucleotides in DNA is normal, their persistence represents a form of DNA damage. Here, we assess such damage and damage defense to DNA in plastids and mitochondria of maize.
Diwaker Tripathi +2 more
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PLoS ONE, 2020 Ribonucleoside triphosphates are often incorporated into genomic DNA during DNA replication. The accumulation of unrepaired ribonucleotides is associated with genomic instability, which is mediated by DNA topoisomerase 1 (Top1) processing of embedded ...
Ayuna Takeishi +11 more
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50 Years of Giese Reaction – a Personal View
Angewandte Chemie, EarlyView.The Giese‐Reaction celebrates its 50. Birthday, it is a cyclic radical chain reaction centered around C,C‐bond formation in a three component synthesis. This minireview speaks about the birth of the name‐reaction, explains how the complete understanding of its reaction mechanism stimulates new methods in organic synthesis, and why it can proceed in ...
Martin Spichty +4 more
wiley +2 more sources
Angewandte Chemie, EarlyView.Here, we transform this otherwise destructive enzymatic activity into a powerful diagnostic advantage through an RNase I–assisted rolling circle amplification (RI‐RCA) strategy. By integrating controlled RNase I–mediated RNA digestion with circular DNA templates, this approach enables direct and highly sensitive detection of target RNA sequences. Using
Amal Mathai +5 more
wiley +2 more sources
Effect of Processing on Volatile Organic Compounds Formation of Meat—Review
Applied Sciences, 2023 Meat is a rich source of different volatile compounds. The final flavor of meat products depends on the raw material and processing parameters.
Iwona Wojtasik-Kalinowska +5 more
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The Effect of Mitochondrial Dysfunction on Cytosolic Nucleotide Metabolism
Journal of Nucleic Acids, 2010 Several enzymes of the metabolic pathways responsible for metabolism of cytosolic ribonucleotides and deoxyribonucleotides are located in mitochondria.
Claus Desler +2 more
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Divergent prebiotic synthesis of pyrimidine and 8-oxo-purine ribonucleotides
Nature Communications, 2017 While mechanisms have been proposed for the prebiotic nucleotide synthesis, these require separate (and potentially incompatible) routes for pyrimidines and purines.
Shaun Stairs +5 more
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Etheno adducts: from tRNA modifications to DNA adducts and back to miscoding ribonucleotides
Genes and Environment, 2021 Etheno (and ethano) derivatives of nucleic acid bases have an extra 5-membered ring attached. These were first noted as wyosine bases in tRNAs. Some were fluorescent, and the development of etheno derivatives of adenosine, cytosine, and guanosine led to ...
F. Peter Guengerich, Pratibha P. Ghodke
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