Results 71 to 80 of about 38,336 (323)

Scientific Opinion on the safety and efficacy of disodium 5?-ribonucleotides, disodium 5?-guanylate, disodium 5?-inosinate for all animal species and categories [PDF]

, 2014
The flavours included in this assessment are widely present in nature as the building blocks of DNA and RNA. In the absence of any information on the microbial strains or substrates used for the production of the additives, and with little information on
EC (European Commission), EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Ishidate, Ishidate, Janas, Mateo, Nelson, Plakantara   +7 more
core   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Abasic and oxidized ribonucleotides embedded in DNA are processed by human APE1 and not by RNase H2

Nucleic Acids Research, 2017
Ribonucleoside 5′-monophosphates (rNMPs) are the most common non-standard nucleotides found in DNA of eukaryotic cells, with over 100 million rNMPs transiently incorporated in the mammalian genome per cell cycle.
M. Malfatti, S. Balachander, G. Antoniali, Kyung Duk Koh, C. Saint-Pierre, D. Gasparutto, H. Chon, R. Crouch, F. Storici, G. Tell   +9 more
semanticscholar   +1 more source

Dual targeting of MIR-208 and MIR-499 in the treatment of cardiac disorders [PDF]

, 2014
The present invention provides a method of treating or preventing cardiac disorders in a subject in need thereof by inhibiting the expression or function of both miR-499 and miR-208 in the heart cells of the subject. In particular, specific protocols for
Olson, Eric S., van Rooij, Eva
core   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair

Nature Communications, 2016
Oxidative stress is a common source of DNA damage and is repaired by the base excision repair machinery, including polymerase beta. Here the authors find that polymerase beta, and to a lesser extent lambda, can mistakenly incorporate ribonucleotides ...
Emmanuele Crespan, Antonia Furrer, Marcel Rösinger, Federica Bertoletti, Elisa Mentegari, Giulia Chiapparini, Ralph Imhof, Nathalie Ziegler, Shana J. Sturla, Ulrich Hübscher, Barbara van Loon, Giovanni Maga   +11 more
doaj   +1 more source

Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase

Nature Communications, 2023
RNase H2 removes mutagenic rNMPs from genomic DNA. The authors demonstrate how nicked rNMPs are repaired when they are encountered in S phase. This study unveils genetic interactions that could potentially be exploited in RNase H2-deficient pathologies.
Natalie Schindler, Matthias Tonn, Vanessa Kellner, Jia Jun Fung, Arianna Lockhart, Olga Vydzhak, Thomas Juretschke, Stefanie Möckel, Petra Beli, Anton Khmelinskii, Brian Luke   +10 more
doaj   +1 more source

HAMR: High-Throughput Annotation of Modified Ribonucleotides.

Methods in molecular biology, 2018
Ribonucleotides can be decorated with over 100 types of covalent chemical modifications. These modifications change the structure, function, and catalytic activity of RNAs, forming a layer of posttranscriptional regulation termed the epitranscriptome ...
Lee E. Vandivier, Zachary D Anderson, B. Gregory   +2 more
semanticscholar   +1 more source

Role of RNase H1 in DNA repair: Removal of single ribonucleotide misincorporated into DNA in collaboration with RNase H2 [PDF]

, 2015
Several RNases H1 cleave the RNA-DNA junction of Okazaki fragment-like RNA-DNA/DNA substrate. This activity, termed 3’-junction ribonuclease (3’-JRNase) activity, is different from the 5’-JRNase activity of RNase H2 that cleaves the 5’-side of the ...
Kanaya, Eiko, Kanaya, Shigenori, Tannous, Elias   +2 more
core   +2 more sources

Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

Molecular Oncology, EarlyView.
In this research, we analyzed the relationship between gut microbiota and tumor. We discovered that both subcutaneous and metastatic tumors would alter the composition and metabolic function of gut microbiota. Meanwhile, fecal microbiota transplantation also indicated the anti‐tumor role of the gut microbiota, revealing the crosstalk between tumor and ...
Siyuan Zhang, Haimei Wen, Ying Chen, Jingya Ning, Di Hu, Yujiao Dong, Chenyu Yao, Bo Yuan, Shuanying Yang   +8 more
wiley   +1 more source