Results 121 to 130 of about 556,680 (355)
PLoS ONE, 2015 Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is ...
Chunxiao Yang +3 more
doaj +1 more source
The architecture of mammalian ribosomal protein promoters
BMC Evolutionary Biology, 2005 Background Mammalian ribosomes contain 79 different proteins encoded by widely scattered single copy genes. Coordinate expression of these genes at transcriptional and post-transcriptional levels is required to ensure a roughly equimolar accumulation of ...
Perry Robert P
doaj +1 more source
Advanced Healthcare Materials, EarlyView.Membrane vesicles derived from the probiotic Lacticaseibacillus casei BL23 demonstrate antimicrobial properties against Escherichia coli and a potential biological effect in improving the overall survival of C. elegans infected with Pseudomonas aeruginosa. These vesicles stimulated immune responses in primary cells without causing toxicity. Our results
Cecilia L. D'Antoni +11 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.Osteoporosis from long‐term glucocorticoid (GIOP) use elevates susceptibility to fracture. This study shows GCs impair ascorbic acid (AA) metabolism in osteoblasts, collagen synthesis and extracellular matrix integrity. AA enhanced collagen biochemical and mechanical properties and restored osteoblast and endothelial function. These findings underscore
Micaila DE Curtis +19 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.A bone substitute with gentamicin physically precipitated onto the surface of carbonate apatite exhibits prompt drug release, high bactericidal activity, and osteogenic capacity. Efficient antibacterial activity mitigates early postoperative neutrophil accumulation, the status of which may serve as a potential parameter for evaluating the antibacterial
Linghao Xiao +6 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.Antimicrobial peptide (AMP)‐loaded nanocarriers provide a multifunctional strategy to combat drug‐resistant Mycobacterium tuberculosis. By enhancing intracellular delivery, bypassing efflux pumps, and disrupting bacterial membranes, this platform restores phagolysosome fusion and macrophage function.
Christian S. Carnero Canales +11 more
wiley +1 more source
Genotype‐phenotype associations in individuals with Diamond Blackfan anaemia
eJHaemIntroduction Diamond Blackfan anaemia (DBA) is a rare disorder characterized by failure of red blood cell production, congenital abnormalities and cancer predisposition, primarily caused by pathogenic germline variants in genes encoding ribosomal ...
D. Matthew Gianferante +6 more
doaj +1 more source
Mammalian cells in culture actively export specific microRNAs [PDF]
, 2009 The discovery of microRNAs (miRNAs) as a new class of regulators of gene expression has triggered an explosion of research, but has left many unanswered questions about how this regulation works and how it is integrated with other regulatory mechanisms ...
David Baxter +4 more
core +1 more source
Advanced Healthcare Materials, EarlyView.HUCMSC‐Apo‐mvs enhance peripheral nerve repair by modulating the inflammatory microenvironment (IME), primarily through coordinated actions on three functional cells. They recruit macrophages and promote their polarization from pro‐inflammatory M1 to anti‐inflammatory M2 phenotypes, increasing secretion of IL‐10 and VEGF.
Haolin Liu +21 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.This work presents ARC‐3D, a soft 3D model that recreates how brain support cells, called astrocytes, react to oxidative stress. The system visualizes rapid calcium changes and inflammatory signals, and shows how the drug KDS12025 can protect cells from damage. ARC‐3D offers a simple, reliable way to study early drivers of brain inflammation.
Ju‐Kang Kim +6 more
wiley +1 more source