Results 1 to 10 of about 1,566,471 (276)

Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits. [PDF]

Nucleic Acids Research, 2017
In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation.
Blaha, Gregor M, Conn, Adam B, Diggs, Stephen, Fan, Haitian, Gamper, Howard B, Hahm, Joseph, Hou, Ya-Ming, O'Leary, Seán E, Wang, Yinsheng, Williams, Preston B   +9 more
core   +2 more sources

Massively parallel sequencing of human urinary exosome/microvesicle RNA reveals a predominance of non-coding RNA. [PDF]

PLoS ONE, 2014
Intact RNA from exosomes/microvesicles (collectively referred to as microvesicles) has sparked much interest as potential biomarkers for the non-invasive analysis of disease.
Kevin C Miranda, Daniel T Bond, Joshua Z Levin, Xian Adiconis, Andrey Sivachenko, Carsten Russ, Dennis Brown, Chad Nusbaum, Leileata M Russo   +8 more
doaj   +5 more sources

Structural basis of ribosomal RNA transcription regulation

Nature Communications, 2021
Ribosomal RNA (rRNA) expression is regulated at the initiation stage of RNA synthesis. Here, the authors report cryo-EM structures of E. coli RNA polymerase and rRNA promoter complex with DksA/ppGpp on the way to open complex formation, identifying key ...
Yeonoh Shin, M. Zuhaib Qayyum, Danil Pupov, Daria Esyunina, Andrey Kulbachinskiy, Katsuhiko S. Murakami   +5 more
doaj   +2 more sources

Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction [PDF]

BMC Genomics, 2018
Background Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly ...
Zachary T. Herbert, Jamie P. Kershner, Vincent L. Butty, Jyothi Thimmapuram, Sulbha Choudhari, Yuriy O. Alekseyev, Jun Fan, Jessica W. Podnar, Edward Wilcox, Jenny Gipson, Allison Gillaspy, Kristen Jepsen, Sandra Splinter BonDurant, Krystalynne Morris, Maura Berkeley, Ashley LeClerc, Stephen D. Simpson, Gary Sommerville, Leslie Grimmett, Marie Adams, Stuart S. Levine   +20 more
doaj   +3 more sources

Folding molecular origami from ribosomal RNA [PDF]

Journal of Nanobiotechnology
Approximately 80 percent of the total RNA in cells is ribosomal RNA (rRNA), making it an abundant and inexpensive natural source of long, single-stranded nucleic acid, which could be used as raw material for the fabrication of molecular origami.
Anastasia Shapiro, Noah Joseph, Nadav Mellul, Almogit Abu-Horowitz, Boaz Mizrahi, Ido Bachelet   +5 more
doaj   +2 more sources

Probing complex RNA structures by mechanical force [PDF]

European Physical Journal E 12 (2003) 605-615, 2003
RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by solely invoking successive openings of native helices.
A. Xayaphoummine, Allemand, Cluzel, Cocco, D. Chatenay, Essevaz-Roulet, Evers, Fersht, Fontana, Gerland, Gutell, H. Isambert, Isambert, J-F. Léger, J. Robert, Kellermayer, Liphardt, Lubensky, Léger, Mathews, Montanari, Nowotny, Onoa, Powers, Rief, Rief, Rief, S. Harlepp, Smith, Svoboda, T. Marchal, Treiber, Treiber, Wimberly, Woodson, Xayaphoummine, Yusupov   +36 more
arxiv   +5 more sources

Pre-Ribosomal RNA Processing in Human Cells: From Mechanisms to Congenital Diseases

Biomolecules, 2018
Ribosomal RNAs, the most abundant cellular RNA species, have evolved as the structural scaffold and the catalytic center of protein synthesis in every living organism.
Maxime Aubert, Marie-Françoise O’Donohue, Simon Lebaron, Pierre-Emmanuel Gleizes   +3 more
doaj   +2 more sources

RNA polymerase I mutant affects ribosomal RNA processing and ribosomal DNA stability [PDF]

RNA Biology
Transcription is a major contributor to genomic instability. The ribosomal RNA (rDNA) gene locus consists of a head-to-tail repeat of the most actively transcribed genes in the genome.
Christophe Normand, Christophe Dez, Lise Dauban, Sophie Queille, Sarah Danché, Sarra Abderrahmane, Frederic Beckouet, Olivier Gadal   +7 more
doaj   +2 more sources

Dual RNA-seq in filarial nematodes and Wolbachia endosymbionts using RNase H based ribosomal RNA depletion [PDF]

Frontiers in Microbiology
Lymphatic filariasis is caused by parasitic nematodes and is a leading cause of disability worldwide. Many filarial worms contain the bacterium Wolbachia as an obligate endosymbiont.
Lindsey J. Cantin, Vanessa Gregory, Laura N. Blum, Jeremy M. Foster   +3 more
doaj   +2 more sources

Evidence for further non-coding RNA genes in the fungal rDNA region [PDF]

MycoKeys, 2022
Non-coding RNA (ncRNA) genes play important, but incompletely understood, roles in various cellular processes, notably translation and gene regulation.
Magnus Alm Rosenblad, Ellen Larsson, Arttapon Walker, Naritsada Thongklang, Christian Wurzbacher, R. Henrik Nilsson   +5 more
doaj   +3 more sources