Results 61 to 70 of about 1,566,471 (276)
Ribosomal history reveals origins of modern protein synthesis. [PDF]
The origin and evolution of the ribosome is central to our understanding of the cellular world. Most hypotheses posit that the ribosome originated in the peptidyl transferase center of the large ribosomal subunit.
Ajith Harish, Gustavo Caetano-Anollés
doaj +1 more source
Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription [PDF]
A method for the isolation of genomic fragments of RNA virus based on cDNA representational difference analysis (cDNA RDA) was developed. cDNA RDA has been applied for the subtraction of poly(A)(+) RNAs but not for poly(A)(−) RNAs, such as RNA virus ...
Endoh, Daiji+7 more
core +2 more sources
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.
Although the applicability of small subunit ribosomal RNA (16S rRNA) sequences for bacterial classification is now well accepted, the general use of these molecules has been hindered by the technical difficulty of obtaining their sequences. A protocol is
D. Lane+5 more
semanticscholar +1 more source
Stochastic kinetics of ribosomes: single motor properties and collective behavior [PDF]
Synthesis of protein molecules in a cell are carried out by ribosomes. A ribosome can be regarded as a molecular motor which utilizes the input chemical energy to move on a messenger RNA (mRNA) track that also serves as a template for the polymerization of the corresponding protein.
arxiv +1 more source
Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ...
U. Edwards+4 more
semanticscholar +1 more source
Ribosomal translocation catalyzed by EF-G hydrolyzing GTP entails multiple conformational changes of ribosome and positional changes of tRNAs and mRNA in the ribosome.
Ping Xie
doaj +1 more source
Three out of the seven ribosomal RNA operons in Escherichia coli end in dual terminator structures. Between the two terminators of each operon is a short sequence that we report here to be an sRNA gene, transcribed as part of the ribosomal RNA primary ...
Thomas Søndergaard Stenum+5 more
doaj +1 more source
Modeling of Ribosome Dynamics on a ds-mRNA under an External Load [PDF]
Protein molecules in cells are synthesized by macromolecular machines called ribosomes. According to recent experimental data, we reduce the complexity of the ribosome and propose a model to express its activity in six main states. Using our model, we study the translation rate in different biological relevant situations in the presence of external ...
arxiv +1 more source
Magnification of Genes Coding for Ribosomal RNA in Saccharomyces cerevisiae [PDF]
When a strain of Saccharomyces cerevisiae monosomic for chromosome I and initially deficient for 25% of the genes coding for ribosomal RNA is repeatedly subcultured, the number of these genes increases to and remains stable at the number in the wild type.
Halvorson, Harlyn O., Kaback, David B.
core
With a stepwise degradation and terminal labeling procedure the 3'-terminal sequence of E. coli 16S ribosomal RNA is shown to be Pyd-A-C-C-U-C-C-U-U-A(OH). It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal
J. Shine, L. Dalgarno
semanticscholar +1 more source