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Identification of Yeast Protein Sequences With Similarity to the ESCRT‐III Protein Snf7
ABSTRACT Endosomal sorting complex required for transport (ESCRT‐III) is a membrane remodeling complex involved in a large number of cellular processes. It appears to perform an essential function in eukaryotes, since to date no eukaryotic organism completely devoid of ESCRT‐III has been found.
Thomas Brune, Ralf Kölling
wiley +1 more source
We present cryoBN‐PAGE, a method that enables blue native polyacrylamide gel electrophoresis analysis of mitochondrial respiratory complexes and supercomplexes directly from whole yeast cells using cryogenic milling. This approach eliminates the need for mitochondrial isolation, reducing time and cost while preserving native protein complexes.
Lizeth J. Camacho Lopez +3 more
wiley +1 more source
Ribosome profiling reveals the role of yeast RNA-binding proteins Cth1 and Cth2 in translational regulation. [PDF]
Barlit H +8 more
europepmc +1 more source
Widespread stable noncanonical peptides identified by integrated analyses of ribosome profiling and ORF features. [PDF]
Yang H, Li Q, Stroup EK, Wang S, Ji Z.
europepmc +1 more source
Riboseq-flow: A streamlined, reliable pipeline for ribosome profiling data analysis and quality control. [PDF]
Iosub IA, Wilkins OG, Ule J.
europepmc +1 more source
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2022
Selective Ribosome Profiling (SeRP) is an emerging methodology, developed to capture cotranslational interactions in vivo. To date, SeRP is the only method that can directly capture, in near-codon resolution, ribosomes in action. Thus, SeRP allows us to study the mechanisms of protein synthesis and the network of protein-protein interactions that are ...
Hagit, Bar-Yosef +3 more
openaire +2 more sources
Selective Ribosome Profiling (SeRP) is an emerging methodology, developed to capture cotranslational interactions in vivo. To date, SeRP is the only method that can directly capture, in near-codon resolution, ribosomes in action. Thus, SeRP allows us to study the mechanisms of protein synthesis and the network of protein-protein interactions that are ...
Hagit, Bar-Yosef +3 more
openaire +2 more sources

