Results 241 to 250 of about 47,955 (276)

In vivo single-cell ribosome profiling reveals cell-type-specific translational programs during aging

Duré C, Ghoshdastider U, Weber R, Valdivia-Francia F, Renz PF, Khandekar A, Sella F, Hyams K, Taborsky D, Yigit M, Ormiston M, Yamahachi H, Levesque M, Ellis S, Sendoel A.   +14 more
europepmc   +1 more source

An in vitro assay of MCTS1-DENR-dependent re-initiation and ribosome profiling uncover the activity of MCTS2 and distinct function of eIF2D

Meurs R, De Matos M, Bothe A, Guex N, Weber T, Teleman AA, Ban N, Gatfield D.   +7 more
europepmc   +1 more source

Transcriptome-wide measurement of ribosomal occupancy by ribosome profiling

Methods, 2015
Gene expression profiling provides a tool to analyze the internal states of cells or organisms, and their responses to perturbations. While global measurements of mRNA levels have thus been widely used for many years, it is only through the recent development of the ribosome profiling technique that an analogous examination of global mRNA translation ...
Florian Aeschimann, Andreas Arnold, Christoph Dieterich   +2 more
exaly   +6 more sources

A snapshot of translation in Mycobacterium tuberculosis during exponential growth and nutrient starvation revealed by ribosome profiling [PDF]

Cell Reports, 2021
Mycobacterium tuberculosis, which causes tuberculosis, can undergo prolonged periods of non-replicating persistence in the host. The mechanisms underlying this are not fully understood, but translational regulation is thought to play a role.
Elizabeth B Sawyer, Jody Phelan, Taane G Clark   +2 more
exaly   +2 more sources

Profiling ribosome dynamics [PDF]

Nature Reviews Molecular Cell Biology, 2016
Eytan Zlotorynski, Zlotorynski Eytan
exaly   +2 more sources

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Purification of Ribosome-Nascent-Chain Complex for Ribosome Profiling and Selective Ribosome Profiling

2022
Selective Ribosome Profiling (SeRP) is an emerging methodology, developed to capture cotranslational interactions in vivo. To date, SeRP is the only method that can directly capture, in near-codon resolution, ribosomes in action. Thus, SeRP allows us to study the mechanisms of protein synthesis and the network of protein-protein interactions that are ...
Hagit, Bar-Yosef, Johannes, Venezian, Kevin, Klann, Ayala, Shiber   +3 more
openaire   +2 more sources

Analysis of Ribosome Profiling Data

2022
Ribosome profiling methods are based on high-throughput sequencing of ribosome-protected mRNA footprints and allow to study in detail translational changes. Bioinformatic and statistical tools are necessary to analyze sequencing data. Here, we describe our developed methods for a fast and reliable quality control of ribosome profiling data, to ...
Carine Legrand, Khanh Dao Duc, Francesca Tuorto   +2 more
openaire   +2 more sources

Ribosome profiling in bacteria [PDF]

Nature Methods, 2012
exaly   +2 more sources

Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling

2021
The emergence of ribosome profiling as a tool for measuring the translatome has provided researchers with valuable insights into the post-transcriptional regulation of gene expression. Despite the biological insights and technical improvements made since the technique was initially described by Ingolia et al. (Science 324(5924):218-223, 2009), ribosome
Alexander L, Cope, Sangeevan, Vellappan, John S, Favate, Kyle S, Skalenko, Srujana S, Yadavalli, Premal, Shah   +5 more
openaire   +2 more sources