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Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling
2021The emergence of ribosome profiling as a tool for measuring the translatome has provided researchers with valuable insights into the post-transcriptional regulation of gene expression. Despite the biological insights and technical improvements made since the technique was initially described by Ingolia et al. (Science 324(5924):218-223, 2009), ribosome
Alexander L, Cope +5 more
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2017
Ribosome profiling (also known as Ribo-seq) provides a genome-wide, high-resolution, and quantitative accounting of mRNA segments that are occupied by ribosomes in vivo. The method has been used to address numerous questions in bacteria, yeast, and metazoa, but its application to questions in plant biology is just beginning.
Prakitchai, Chotewutmontri +3 more
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Ribosome profiling (also known as Ribo-seq) provides a genome-wide, high-resolution, and quantitative accounting of mRNA segments that are occupied by ribosomes in vivo. The method has been used to address numerous questions in bacteria, yeast, and metazoa, but its application to questions in plant biology is just beginning.
Prakitchai, Chotewutmontri +3 more
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Tissue-Specific Ribosome Profiling in Drosophila
2021Robust mechanisms exist that serve to dynamically regulate the translation of mRNA into proteins across heterogeneous tissues. These processes ensure timely generation of proteins in quantities that scale with the demands of specific cell types.
Xun, Chen, Dion, Dickman
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Ribosome Profiling in Trypanosomatids
2019Ribosomes are the machinery responsible for reading mRNAs and translating them into proteins. The ribosome profiling approach is based on high-throughput sequencing of ribosome-protected mRNAs. RNAs not harboring ribosomes are removed by nuclease digestion leaving the so-called ribosome "footprints." The purified "footprint" RNA molecules are processed
Amelie J, Kraus, Raúl O, Cosentino
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Transcriptome-wide measurement of ribosomal occupancy by ribosome profiling
Methods, 2015Gene expression profiling provides a tool to analyze the internal states of cells or organisms, and their responses to perturbations. While global measurements of mRNA levels have thus been widely used for many years, it is only through the recent development of the ribosome profiling technique that an analogous examination of global mRNA translation ...
Aeschimann, F. +4 more
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Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling
2016Recent technological advances (e.g., microarrays and massively parallel sequencing) have facilitated genome-wide measurement of many aspects of gene regulation. Ribosome profiling is a high-throughput sequencing method used to measure gene expression at the level of translation.
Pieter, Spealman +4 more
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Choice of Ribonucleases for Ribosome Profiling Experiments
2021In the past 10 years, standard transcriptome sequencing protocols were optimized so well that no prior experience is required to prepare the sequencing library. Often, all enzymatic steps are designed to work in the same reaction tube minimizing handling time and reducing human errors. Ribosome profiling stands out from these methods.
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Illuminating translation with ribosome profiling spectra
Nature Methods, 2016Software based on the spectral analysis of ribosome profiling improves the detection of translated segments in RNA molecules.
Pavel V Baranov, Audrey M Michel
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Ribosome Profiling of Vaccinia Virus-Infected Cells
2019Ribosome profiling is a method that determines genome-wide mRNA translation through measuring ribosome-protected mRNA fragments by deep sequencing. This method can be used to quantify gene expression at the translational level and precisely pinpoint ribosome loading onto mRNA with codon-level resolution.
Yongquan, Lin +2 more
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