Results 241 to 250 of about 32,451 (274)

Modification of ribulose bisphosphate carboxylase from Rhodospirillumrubrum with tetranitromethane

Biochemical and Biophysical Research Communications, 1979
Abstract Ribulose bisphosphate carboxylase from Rhodospirillum rubrum is inactivated by low concentrations of tetranitromethane. Addition of the substrate ribulose 1,5-bisphosphate and preincubation with Mg+2 and HCO 3 − both protect against inactivation.
P D, Robison, F R, Tabita
openaire   +2 more sources

Regulation of activation of ribulose bisphosphate carboxylase from Pseudomonasoxalaticus

Biochemical and Biophysical Research Communications, 1978
Abstract 6-phosphogluconate, potentiated activation of ribulose bisphosphate carboxylase from Pseudomonas oxalaticus whereas fructose-1,6-bisphosphate inhibited activation and fructose-6-phosphate had no effect. The presence of 1 mM 6-phosphogluconate during activation reduced the Kact for Mg2+ from 1.4 mM to approximately 0.2 mM.
V B, Lawlis, G L, Gordon, B A, McFadden
openaire   +2 more sources

Some Mechanistic Aspects of Ribulose Bisphosphate Carboxylase

1984
Water is involved in the carboxylase reaction in 2 distinct ways (Fig 1.) Besides its stoichiometric involvement, it hydrates both substrates. While carbon dioxide has long been recognised as the active species, no information is yet available on how the enzyme handles the geM-diol form of RuBP.
George H. Lorimer, John Pierce, Steven Gutteridge, John V. Schloss   +3 more
openaire   +1 more source

Comparative Biochemistry of Ribulose Bisphosphate Carboxylase in Higher Plants

1978
The agronomically important aspects of the comparative biochemistry of RuBP carboxylase are locating a natural enzyme, creating a mutant enzyme, or identifying compounds which differentially alter the enzyme so as to allow CO2 to be fixed more efficiently or O2 to be fixed less efficiently.
W L, Ogren, L D, Hunt
openaire   +2 more sources

Stoichiometry in the assay of ribulose bisphosphate oxygenase and carboxylase

Analytical Biochemistry, 1982
Abstract Complete stoichiometry of the reaction catalyzed by ribulose 1,5-bisphosphate (RuBP) oxygenase from spinach and Rhodospirillum rubrum has been determined. Before initiation and after termination, RuBP has been measured either by release of equimolar orthophosphate at 25°C in the presence of 1 n NaOH or by complete carboxylation using 14CO2
K, Purohit, B A, McFadden, A, Saluja
openaire   +2 more sources

Reversible dissociation and conformational stability of dimeric ribulose bisphosphate carboxylase

Biochemistry, 1993
Dimer-monomer dissociation of ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum was investigated using hydrostatic pressure in the range 1-2 kbar to promote dissociation. Intrinsic fluorescence emission and polarization, along with the polarization of the fluorescence of single-labeled AEDANS conjugates, were used to follow the ...
L, Erijman, G H, Lorimer, G, Weber
openaire   +2 more sources

Ribulose bisphosphate carboxylase—oxygenase: its role in photosynthesis

Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1986
Synthesis of triose phosphate by the chloroplast requires three substrates: light, CO2and orthophosphate (Pi). In the response of the rate of carbon assimilation to the concentration of CO2, the kinetic properties of RuBP carboxylase-oxygenase (Rubisco) constitute the main limitation at low CO2concentrations, while at higher concentrations of CO2the ...
openaire   +1 more source

The Active Site of Ribulose Bisphosphate Carboxylase / Oxygenase

1990
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) has attracted a lot of interest due to its central role in the carbon metabolism of plants and photosynthetic microorganisms (for a review see (1)). The dual function of this enzyme, catalyzing the primary steps in both photosynthetic carbon dioxide fixation and photorespiration, makes it a ...
G. Schneider, Y. Lindqvist, T. Lundqvist
openaire   +1 more source

Radiochemical assay of ribulose bisphosphate carboxylase

Biochemical Education, 1989
Carl S. Pike, Joseph A. Berry
openaire   +1 more source

Fibroblast pyruvate carboxylase is required for collagen production in the tumour microenvironment

Nature Metabolism, 2021
Simon Schwörer, Natalya N Pavlova, Bryan King   +2 more
exaly