Results 191 to 200 of about 10,427 (208)
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1988
Bacterial and plant toxins composed of different protein subunits with diverse biological functions have provided an investigational focal point for researchers in many areas of specialization. Potent catalytic toxins have been linked to monoclonal antibodies (MoAb) for antibody directed cell targeting.
Daniel A. Vallera, Dorothea E. Myers
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Bacterial and plant toxins composed of different protein subunits with diverse biological functions have provided an investigational focal point for researchers in many areas of specialization. Potent catalytic toxins have been linked to monoclonal antibodies (MoAb) for antibody directed cell targeting.
Daniel A. Vallera, Dorothea E. Myers
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Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli
Protein Expression and Purification, 2004Ricin and its A chains can be used to conjugate with monoclonal antibodies to prepare immunotoxins. Ricin A chain (RTA) and its modification RTA-KDEL (ER-retrieval signal) were expressed with the pKK223.3 system in Escherichia coli under control of a tac promoter.
Jinbiao Zhan +4 more
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Studies on the galactose-binding site of ricin and the hybrid toxin man6P-ricin
Cell, 1981N-acetylimidazole (NAI) was used to O-acetylate the plant seed toxin ricin. O-acetylation of one to two tyrosine residues per molecule of ricin inhibited ricin binding to Sepharose 4B and decreased toxicity by 90% in a protein synthesis inhibition assay in HeLa cells. Lactose, known to block the binding site on the ricin B subunit, protected ricin from
Gary J. Murray +2 more
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2010
Ricin is a protein toxin produced by the castor bean plant, Ricinus communis. Since its discovery in the late 1800s, it has been recognized as one of the most potent plant toxins in existence. While all parts of the plant contain ricin, the castor bean seed contains the highest concentration ranging from approximately 1 to 5% by weight.
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Ricin is a protein toxin produced by the castor bean plant, Ricinus communis. Since its discovery in the late 1800s, it has been recognized as one of the most potent plant toxins in existence. While all parts of the plant contain ricin, the castor bean seed contains the highest concentration ranging from approximately 1 to 5% by weight.
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Revue de botanique appliquée et d'agriculture coloniale, 1930
Trochain Jean. Le Ricin.. In: Revue de botanique appliquée et d'agriculture coloniale, 10ᵉ année, bulletin n°105, mai 1930. pp. 299-308.
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Trochain Jean. Le Ricin.. In: Revue de botanique appliquée et d'agriculture coloniale, 10ᵉ année, bulletin n°105, mai 1930. pp. 299-308.
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Biochemical and Biophysical Research Communications, 1984
To analyze the influence of ricin B-chain on the toxicity of hybrid-protein conjugates, the rate of cellular uptake of conjugates, and the rate at which ricin A-chain (RTA) is delivered to the cytoplasm, we have constructed toxic hybrid proteins consisting of epidermal growth factor (EGF) coupled in disulfide linkage either to ricin or to RTA.
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To analyze the influence of ricin B-chain on the toxicity of hybrid-protein conjugates, the rate of cellular uptake of conjugates, and the rate at which ricin A-chain (RTA) is delivered to the cytoplasm, we have constructed toxic hybrid proteins consisting of epidermal growth factor (EGF) coupled in disulfide linkage either to ricin or to RTA.
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Insertional mutagenesis of ricin A chain: a novel route to an anti-ricin vaccine
Vaccine, 2004The insertion of a specific 25-residue internal peptide into ricin toxin A chain (RTA) reduced the catalytic activity of this protein approximately 300-fold. Directed proteolytic cleavage of the peptide insert essentially restored catalytic activity of the resulting two peptide A chain to normal levels.
J. Michael Lord +6 more
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Journal of Toxicology: Toxin Reviews, 2004
Ricin is a potent protein toxin derived from the beans of the castor plant (Ricinus communis). Castor beans are found in many parts of the world, and the toxin can be produced simply. Ricin's significance as a potential terrorist weapon relates in part to its wide availability and ease of extraction.
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Ricin is a potent protein toxin derived from the beans of the castor plant (Ricinus communis). Castor beans are found in many parts of the world, and the toxin can be produced simply. Ricin's significance as a potential terrorist weapon relates in part to its wide availability and ease of extraction.
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Ricin-Holotoxin-Based Vaccines: Induction of Potent Ricin-Neutralizing Antibodies
2016Ricin is one of the most potent and lethal toxins known to which there is no available antidote. Currently, the most promising therapy is based on neutralizing antibodies elicited by active vaccination or given passively. Here, detailed protocols are provided for the production of two ricin holotoxin-based vaccines: monomerized subunit-based vaccine ...
Tamar Sabo, Chanoch Kronman, Ohad Mazor
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