Production and characterization of monoclonal antibodies to Rickettsia rickettsii [PDF]
Five mouse ascitic fluids (MAFs) containing monoclonal antibody to Rickettsia rickettsii were produced from three original fusions by murine hybridoma technology. The five MAFs were fractionated and purified; each contained monoclonal antibody of the immunoglobulin G2a subclass.
James V. Lange, David H. Walker
openalex +3 more sources
Selective fragmentation of the trans-Golgi apparatus by Rickettsia rickettsii [PDF]
Karin Aistleitner +3 more
openalex +3 more sources
Pathogenic rickettsiae encode a secreted lipase that facilitates intracytosolic colonization in host cells. [PDF]
Key cellular processes for the rickettsial obligate intracellular lifestyle, including internalization by phagocytosis, regulation of intracellular trafficking, and evasion of lysosomal destruction to establish an intracytosolic replication niche, remain
Mohammad Sadik +7 more
doaj +2 more sources
Absorption of Rickettsia rickettsii antibodies by Rickettsia rickettsii antigens in four diagnostic tests [PDF]
Immunoabsorption profiles were determined for Rickettsia rickettsii antigens used in four confirmatory tests for detection of antibodies to Rocky Mountain spotted fever. A human serum reactive in the four tests was absorbed with each test antigen and then reexamined by all four tests.
K E, Hechemy +4 more
openaire +2 more sources
Laboratory Maintenance of Rickettsia rickettsii [PDF]
AbstractThis unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium Rickettsia rickettsii, including propagation in mammalian cell cultures, as well as isolation, counting, and storage procedures. Regulations for working with R. rickettsii in biosafety level 3 containment are also discussed. Curr.
Nicole C, Ammerman +2 more
openaire +2 more sources
Genomic Comparison of Virulent Rickettsia rickettsii Sheila Smith and Avirulent Rickettsia rickettsii Iowa [PDF]
ABSTRACT Rickettsia rickettsii is an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. To identify genes involved in the virulence of R. rickettsii , the genome of an avirulent strain, R.
Damon W, Ellison +5 more
openaire +2 more sources
Detection of fibrils associated with Rickettsia rickettsii [PDF]
The ultrastructural appearance of the "halozone" formed at the interface between the spotted fever agent Rickettsia rickettsii and the cytoplasm of persistently infected cultured vole cells (Microtus pennsylvanicus) was studied by transmission electron microscopy. In sections of epoxy-embedded specimens stained with uranyl acetate and lead citrate, the
W J, Todd, W, Burgdorfer, G P, Wray
openaire +2 more sources
Genetic sequencing of a 1944 Rocky Mountain spotted fever vaccine
Rocky Mountain spotted fever (RMSF) is a rapidly progressive and often fatal tick-borne disease caused by Rickettsia rickettsii. Its discovery and characterization by Howard Ricketts has been hailed as a remarkable historical example of detection and ...
Yongli Xiao +5 more
doaj +1 more source
Brazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp.
Fernanda Silva Fortes +7 more
doaj +1 more source
Detecção de anticorpos anti-Rickettsia rickettsii em cães residentes em área negligenciada no município de São Paulo, SP, Brasil [PDF]
RESUMO A febre maculosa brasileira (FMB), descrita inicialmente nos Estados Unidos como febre maculosa das Montanhas Rochosas, é uma antropozoonose relatada apenas no continente americano e causada pela bactéria Rickettsia rickettsii.
Z.Ê.S. Souza +9 more
doaj +1 more source

