Results 51 to 60 of about 539,954 (312)

Strategies for robust renovation of residential buildings in Switzerland

Nature Communications
Building renovation is urgently required to reduce the environmental impact associated with the building stock. Typically, building renovation is performed by envelope insulation and/or changing the fossil-based heating system.
Alina Galimshina, Maliki Moustapha, Alexander Hollberg, Sébastien Lasvaux, Bruno Sudret, Guillaume Habert   +5 more
doaj   +1 more source

Development of a digital twin operational platform using Python Flask

Data-Centric Engineering, 2022
The digital twin concept has developed as a method for extracting value from data, and is being developed as a new technique for the design and asset management of high-value engineering systems such as aircraft, energy generating plant, and wind ...
Matthew S. Bonney, Marco de Angelis, Mattia Dal Borgo, Luis Andrade, Sandor Beregi, Nidhal Jamia, David J. Wagg   +6 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Editorial for the special issue: “Novel Solutions and Novel Approaches in Operational Research”

Business Systems Research, 2020
This special issue of Business Systems Research (SI of the BSR) is co-published by the Slovenian Society INFORMATIKA – Section for Operational Research (SSI-SOR) and highlights recent advances in Operations Research and Management Science (OR /MS), with ...
Drobne Samo, Dumičić Ksenija, Stirn Lidija Zadnik   +2 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Linear response of the Greenland ice sheet's tidewater glacier terminus positions to climate

Journal of Glaciology, 2021
Gaining knowledge of tidewater glacier (TWG) margin evolution, solid ice flux and their responses to climate over large spatio-temporal scales provides valuable context for the projection of future Greenland ice sheet (GrIS) change.
Dominik Fahrner, James M. Lea, Stephen Brough, Douglas W. F. Mair, Jakob Abermann   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Beliefs about EU 2030 climate commitments across public, expert, and policy audiences

Environmental Research Letters
The European Union (EU) has adopted ambitious climate policies to reduce greenhouse gas emissions by 55% by 2030. While technical, economic, and political feasibility have been widely analyzed, far less is known about a critical enabling factor: whether ...
Loïc Berger, Thibault Richard, Johannes Emmerling, Thomas Epper, Nahed Eddai, Mary Sanford, Silvia Pianta, Uyanga Turmunkh, Valentina Bosetti   +8 more
doaj   +1 more source