Results 111 to 120 of about 2,524,971 (285)

Messenger RNA degradation in bacterial cells.

Annual Review of Genetics, 2014
mRNA degradation is an important mechanism for controlling gene expression in bacterial cells. This process involves the orderly action of a battery of cellular endonucleases and exonucleases, some universal and others present only in certain species ...
Monica P. Hui, P. Foley, J. Belasco
semanticscholar   +1 more source

Intraoral Drug Delivery: Bridging the Gap Between Academic Research and Industrial Innovations

Advanced Functional Materials, EarlyView.
Intraoral drug delivery offers a promising route for systemic and localized therapies, yet challenges such as enzymatic degradation, limited permeability, and microbial interactions hinder efficacy. This figure highlights innovative strategies—mucoadhesive materials, enzyme inhibitors, and permeation enhancers—to overcome these barriers.
Soheil Haddadzadegan, Simona Summonte, Fabrizio Ricci, Matthias Sandmeier, Andreas Bernkop‐Schnürch   +4 more
wiley   +1 more source

Characterizing living ocular bacterial communities and the effects of antibiotic perturbation in house finches

MicrobiologyOpen
DNA‐based methods to measure the abundance and relative abundance of bacterial taxa can be skewed by the presence of dead or transient bacteria. Consequently, the active, functional members of the community may be a small subset of the detected bacterial
Chava L. Weitzman, Dana M. Hawley, Bahman Rostama, Meghan May, Lisa K. Belden   +4 more
doaj   +1 more source

Bacteria‐Derived Extracellular Vesicle as A “Trojan Horse” for Selective M1 Macrophage‐Targeting in A Multi‐Cellular Entanglement Environment

Advanced Functional Materials, EarlyView.
The temporary transition of macrophages from a pro‐inflammatory phenotype of macrophages (M1) to an anti‐inflammatory phenotype of macrophages (M2) is crucial for tissue repair and regeneration processes. Bacterial outer membrane vesicles (OMVs) are utilized as a “trojan horse” for specific M1 macrophage‐targeting and anti‐inflammatory drug delivery ...
Donglin Cai, Zhelun Li, Wendong Gao, Yuqing Mu, Jiaying Liu, Yufeng Zhang, Xiaohan Mei, Jingjing Quan, Lan Xiao, Yin Xiao   +9 more
wiley   +1 more source

Optimized Rapid Amplification of cDNA Ends (RACE) for Mapping Bacterial mRNA Transcripts

BioTechniques, 2000
A simple, efficient and sensitive RACE-based procedure was developed for the determination of unknown 5′ regions from bacterial cDNA. A number of critical modifications were made to the standard RACE method, including the optimization of the RNA ...
Daniel Tillett, Brendan P. Burns, Brett A. Neilan   +2 more
doaj   +1 more source

Structural Basis of Transcription Initiation by Bacterial RNA Polymerase Holoenzyme*

Journal of Biological Chemistry, 2014
Background: Cellular RNA polymerases start transcription by de novo RNA priming. Results: Structures and biochemical studies of initially transcribing complexes elucidate the de novo transcription initiation and early stage of RNA transcription ...
R. Basu, B. Warner, V. Molodtsov, D. Pupov, D. Esyunina, C. Fernández‐Tornero, A. Kulbachinskiy, K. Murakami   +7 more
semanticscholar   +1 more source

Secreted bacterial RNA: an unexplored avenue [PDF]

FEMS Microbiology Letters, 2018
Gradually, it is becoming clear that our well-being depends significantly on the contribution and composition of microorganisms that are associated with us. The majority of human-associated microorganisms are bacteria, which maintain their niche through interactions with the human host and neighboring microorganisms.
openaire   +2 more sources

Transmembrane Activation of Catalysis and Protein Refolding in Synthetic Cells by Enzymes and Nanozymes

Advanced Functional Materials, EarlyView.
Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen, Andreas B. Pedersen, Mireia Casanovas Montasell, Søren Moesgaard, Anja Bille Bohn, Alexander N. Zelikin   +5 more
wiley   +1 more source

RNA-seq reproducibility of Pseudomonas aeruginosa in laboratory models of cystic fibrosis

Microbiology Spectrum
Reproducibility is a fundamental expectation in science and enables investigators to have confidence in their research findings and the ability to compare data from disparate sources, but evaluating reproducibility can be elusive. For example, generating
Rebecca P. Duncan, Gina R. Lewin, Daniel M. Cornforth, Frances L. Diggle, Ananya Kapur, Dina A. Moustafa, Yasmin Hilliam, Jennifer M. Bomberger, Marvin Whiteley, Joanna B. Goldberg   +9 more
doaj   +1 more source

RNA-guided DNA insertion with CRISPR-associated transposases

Science, 2019
Beyond adaptive immunity Prokaryotic CRISPR-Cas systems defend bacterial cells from phage and plasmid infection. Strecker et al. characterized a CRISPR-Cas system that functions beyond adaptive immunity (see the Perspective by Hou and Zhang).
Jonathan Strecker, Alim Ladha, Zachary Gardner, Jonathan L. Schmid-Burgk, K. Makarova, E. Koonin, Feng Zhang   +6 more
semanticscholar   +1 more source