Results 121 to 130 of about 2,944,137 (233)

Characterizing living ocular bacterial communities and the effects of antibiotic perturbation in house finches

MicrobiologyOpen
DNA‐based methods to measure the abundance and relative abundance of bacterial taxa can be skewed by the presence of dead or transient bacteria. Consequently, the active, functional members of the community may be a small subset of the detected bacterial
Chava L. Weitzman, Dana M. Hawley, Bahman Rostama, Meghan May, Lisa K. Belden   +4 more
doaj   +1 more source

RNA-seq reproducibility of Pseudomonas aeruginosa in laboratory models of cystic fibrosis

Microbiology Spectrum
Reproducibility is a fundamental expectation in science and enables investigators to have confidence in their research findings and the ability to compare data from disparate sources, but evaluating reproducibility can be elusive. For example, generating
Rebecca P. Duncan, Gina R. Lewin, Daniel M. Cornforth, Frances L. Diggle, Ananya Kapur, Dina A. Moustafa, Yasmin Hilliam, Jennifer M. Bomberger, Marvin Whiteley, Joanna B. Goldberg   +9 more
doaj   +1 more source

Origin of Life

, 2012
The evolution of life has been a big enigma despite rapid advancements in the fields of biochemistry, astrobiology, and astrophysics in recent years.
A. Gibbons, Ashwini Kumar Lal, C. Bohler, C. Chyba, C. Huber, C.E. Manning, C.J. Salter, D. Penny, D. Warmflash, D.S. Heckman, D.S. Mckay, E.G. Roussel, F. Hoyle, F. Hoyle, F. Hoyle, G.F. Joyce, H.C. Arp, H.C. Urey, J. Li, J. Maddox, J. Postgate, J.B.S. Haldane, J.K. Fredrickson, J.V. Narlikar, J.W. Schopf, K. Kvenvolden, L.-H. Lin, L.E. Orgel, L.J. Richardson, M.T. Mondigan, N.C. Wickramsinghe, P. David, P. Ehrenfreund, P. Senapathy, R. Shapiro, R.D. Lorenz, S Arrhenius, S.J. Mojzsis, S.L. Miller, S.L. Miller, Stofan, T. Brock, T.D. Brack, W. Gilbert, W. Martin, Y.-J. Kuan, Y.-J. Ma   +46 more
core   +1 more source

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

Nature Biotechnology, 2020
P. Vo, Carlotta Ronda, Sanne E. Klompe, Ethan E. Chen, Christopher Acree, Harris H. Wang, S. H. Sternberg   +6 more
semanticscholar   +1 more source

Antibiotic resistance evolved via inactivation of a ribosomal RNA methylating enzyme. [PDF]

, 2016
Modifications of the bacterial ribosome regulate the function of the ribosome and modulate its susceptibility to antibiotics. By modifying a highly conserved adenosine A2503 in 23S rRNA, methylating enzyme Cfr confers resistance to a range of ribosome ...
Fujimori, Danica Galonić, Noda-Garcia, Lianet, Stojković, Vanja, Tawfik, Dan S   +3 more
core  

Stepwise Promoter Melting by Bacterial RNA Polymerase.

Molecules and Cells, 2020
James Chen, C. Chiu, S. Gopalkrishnan, Albert Y. Chen, P. D. Olinares, R. Saecker, Jared T. Winkelman, M. F. Maloney, B. Chait, W. Ross, R. Gourse, E. Campbell, S. Darst   +12 more
semanticscholar   +1 more source

Diagnostic value of DNA or RNA-based metagenomic next-generation sequencing in lower respiratory tract infections

Heliyon
Objectives: We aimed to evaluate and compare the diagnostic performance of RNA-mNGS and DNA-mNGS workflow in bacterial pneumonia, fungal pneumonia and tuberculosis.
Jiafu Song, Suxia Liu, Yongpeng Xie, Chen Zhang, Caiyun Xu   +4 more
doaj   +1 more source

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Science, 2012
M. Jinek, Krzysztof Chylinski, Ines Fonfara, Michael H. Hauer, J. Doudna, E. Charpentier   +5 more
semanticscholar   +1 more source

Unveiling the orchestration: mycobacterial small RNAs as key mediators in host-pathogen interactions

Frontiers in Microbiology
Small RNA (sRNA) molecules, a class of non-coding RNAs, have emerged as pivotal players in the regulation of gene expression and cellular processes.
Rajni Garg, Ishali Manhas, Diksha Chaturvedi   +2 more
doaj   +1 more source

Clinical metagenomics. [PDF]

, 2019
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly moving from research to clinical laboratories. This emerging approach is changing
Chiu, Charles Y, Miller, Steven A
core