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Chromosome architecture affects virulence and competitiveness in <i>Agrobacterium tumefaciens</i> C58. [PDF]
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An RNA regulates iron homeostasis and host mucus colonization in Bacteroides thetaiotaomicron
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Current Protocols in Molecular Biology, 1993
AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
Michael Z. Gilman, K.J. Reddy
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AbstractProcedures for isolating RNA from bacteria involve disruption of the cells, followed by steps to separate the RNA from contaminating DNA and protein. Lysis strategies differ in the protocols presented in this unit, including chemical degradation of Gram‐negative cell walls using sucrose/detergent or lysozyme, and sonication to break open Gram ...
Michael Z. Gilman, K.J. Reddy
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Autodegradation of RNA of bacterial ribosomes
Canadian Journal of Microbiology, 1973The autodegradation capacity of ribosomes, in the presence or absence of EDTA, has been studied in resting and dividing bacteria. This capacity, in the presence of EDTA, was found to be much lower in the ribosomes of log-phase bacteria when compared to stationary phase bacteria. In absence of EDTA, autodegradation was not observed.
C. Gagnon, G. de Lamirande
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Cold Spring Harbor Protocols, 2012
In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
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In this bacterial RNA isolation protocol, an “RNA-protective” treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan.
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On the biosynthesis of bacterial ribosomal RNA
Molecular Biology Reports, 1973By comparison of the fingerprints of 5S and 23S ribosomal RNAs from Bacillus licheniformis with that of the precursor of 23S ribosomal RNA, it can be shown that 5S RNA is not a part of the precursor of 23S ribosomal RNA.
T. J. Stoof, R. J. Planta
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Current Opinion in Structural Biology, 2001
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
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The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
openaire +3 more sources