Results 221 to 230 of about 379,319 (234)

Antisense Catalytic RNAs as Therapeutic Agents

1994
Publisher Summary This chapter presents an overview of well-characterized ribozymes with an emphasis on the problems that remain to be overcome before ribozyme therapy can be made a useful form of medical treatment. The chapter illustrates that many techniques are currently being used to enhance ribozyme activity.
Daniela Castanotto, John J. Rossi, Nava Sarver   +2 more
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Amplification, mutation and selection of catalytic RNA

Gene, 1989
RNA, by virtue of its genotypic and phenotypic properties, is a suitable substrate for molecular evolution in the laboratory. We have developed techniques for the rapid amplification, mutation and selection of catalytic RNA. By combining these techniques in an iterative fashion, we are attempting to construct an RNA-based evolving system. Such a system
openaire   +3 more sources

Catalytic DNA and RNA for Targeting MDR1 mRNA

Nucleosides, Nucleotides and Nucleic Acids, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Valentin V. Vlassov, Mark Lukin, Alya G. Venyaminova, M. Repkova, A. A. Fokina, M. A. Kuznetsova   +5 more
openaire   +4 more sources

Catalytic metallodrugs targeting HCV IRES RNA

Chemical Communications, 2012
A catalytic metallodrug that targets stem-loop IIb of the internal ribosomal entry site (IRES) RNA of hepatitis C virus (HCV) demonstrates enzyme-like turnover with K(M) of 0.85 μM, k(cat) of 0.53 min(-1), and a turnover number of 31.9 for Cu·GGHYrFK-amide (1-Cu), and yielded an antiviral activity (IC(50)) of 0.58 μM in an HCV cellular replicon assay.
James A. Cowan, Seth Bradford
openaire   +3 more sources

Defining the Inside and Outside of a Catalytic RNA Molecule

Science, 1989
Ribozymes are RNA molecules that catalyze biochemical reactions. Fe(II)-EDTA, a solvent-based reagent which cleaves both double- and single-stranded RNA, was used to investigate the structure of the Tetrahymena ribozyme.
Thomas R. Cech, John A. Latham
openaire   +3 more sources

An RNA‐Cleaving Catalytic DNA Accelerated by Freezing

ChemBioChem, 2018
AbstractThe EtNa DNAzyme was isolated during the isopropanol precipitation step of an in vitro selection effort. Although inactive with the intended cofactor, its RNA cleavage activity was observed under a few conditions. With Na+, EtNa was highly active in ∼50 % ethanol, whereas in water, it was highly active with Ca2+.
Wenhu Zhou, Wenhu Zhou, Juewen Liu, Tianmeng Yu   +3 more
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Recent insights on RNA folding mechanisms from catalytic RNA

Cellular and Molecular Life Sciences (CMLS), 2000
Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly.
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Coherent collective modes in catalytic RNA

Zeitschrift f�r Physik B Condensed Matter, 1990
We establish the existence ofФ4-solitons travelling along the backbone of an RNA double stranded helix. Taking advantage of the catalytic activity of RNA in proton transfer events, we device a probe for such coherent solitary-wave excitations: We prove that the density of domain walls is increased by the same phonon excitations which would enhance ...
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RNA catalytic networks [PDF]

Nature Reviews Genetics, 2012
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Cloning Strategies for Catalytic Antisense RNAs [PDF]

, 2003
Mina Tsagris, Martin Tabler
openaire   +2 more sources