Results 211 to 220 of about 180,763 (256)
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RNA decay by messenger RNA interferases.
Methods in enzymology, 2008Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and ...
Christensen-Dalsgaard M +3 more
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The structure of pre-messenger RNA and messenger RNA from erythroid cells
1977Pre-mRNA fractions (greater than 45 S) were characterized by electron microscopy. High salt concentrations (0.2 M ammonium acetate, pH 8) yield linear molecules of different length (0.5--17 micrometer). In 10% of the molecules a compact-nonlinear contour (cn-contour) is detectable at one end.
A M, Ladhoff +3 more
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Radiation sensitivity of messenger RNA
Molecular and General Genetics MGG, 1979Messenger RNA function is inactivated by irradiation with ultraviolet light. A unit length mRNA (in bases) is 2-3 times more sensitive than a unit length of DNA (in base pairs) with respect to the inactivation of template function. These data stem from four experimental systems all of which do not repair DNA: the translation of E.
Ponta, H. +4 more
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The relation of messenger to nucleolar RNA
Experimental Cell Research, 1962Abstract 1. 1. Nucleolar RNA has a vectorial turnover with the dense-particulate part at the origin determining either a centrifugal or a centripetal stable pattern. 2. 2. The centrifugal turnover pattern used in this work lends itself to inhibition studies. 3. 3.
J L, SIRLIN, J, JACOB, K I, KATO
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Hormone inducible messenger RNA
Life Sciences, 1975Abstract Steroid hormones regulate the synthesis of specific proteins in target tissue by controlling the synthesis of the corresponding messenger RNA's. In the chick oviduct, estrogen and progesterone induce the synthesis and accumulation of the specific messenger RNA's for the hormone-induced egg-white proteins, ovalbumin and avidin, respectively ...
S L, Woo, B W, O'Malley
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The Decay of Bacterial Messenger RNA
1996Publisher Summary The many demonstrations that the Escherichia coli (E. coli ) rne gene product (RNase E) is involved in messenger RNA (mRNA) decay have given real impetus to the study of this unusual protein's properties and role. The recent attention given to the polyadenylylation of bacterial mRNAs and the discovery that polyadenylylation ...
D P, Nierlich, G J, Murakawa
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Determinants of messenger RNA stability
Cell, 1987The steady-state levels of functional mRNAs are determined in part by their rates of decay in the cytoplasm. Hence, this process plays an important role in the control of gene expression. Individual mRNA species differ widely with respect to metabolic stability.
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The Isolation of Eukaryotic Messenger RNA
Annual Review of Biochemistry, 1979PERSPECflVES AND SUMMARY 682 GENERAL TECHNIQUES 683 Ribonuclease Inhibition 683 Extraction of Total Cellular RNA 684 Poiy(A) Adsorption Methods 686 Translation Assays 688 cDNA Synthesis and Hybridization 689 ISOLATION OF mRNA 691 Size Fractionation .........
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