Results 41 to 50 of about 1,099,258 (120)
The Spanning Tree Model and the Assembly Kinetics of RNA Viruses [PDF]
arXiv, 2021 Single-stranded (ss) RNA viruses self-assemble spontaneously in solutions that contain the viral RNA genome molecules and the viral capsid proteins. The self-assembly of empty capsids can be understood on the basis of free energy minimization of rather simple models.
arxiv
Systemic delivery of factor IX messenger RNA for protein replacement therapy
Proceedings of the National Academy of Sciences of the United States of America, 2017 Significance Abnormal gene expression is the underlying cause for many pathological states, and restoring normalcy through overexpression or knockdown is a conceptually simple solution.
Suvasini Ramaswamy +7 more
semanticscholar +1 more source
The Spanning Tree Model and the Assembly Kinetics of RNA Viruses [PDF]
arXiv, 2022 Single-stranded (ss) RNA viruses self-assemble spontaneously in solutions that contain the viral RNA genome molecules and viral capsid proteins. The self-assembly of empty capsids can be understood on the basis of free energy minimization. However, during the self-assembly of complete viral particles in the cytoplasm of an infected cell, the viral ...
arxiv
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
European Journal of Biochemistry, 2005 A procedure is described for the large-scale purification of light (L) and heavy (H) chain mRNAs from plasmacytomas produced in mice. Intact RNA is selectively precipitated in high yield from frozen tumors homogenized in 3 M LiCl and 6 M urea.
C. Auffray, F. Rougeon
semanticscholar +1 more source
Deciphering transcriptional dynamics in vivo by counting nascent RNA molecules [PDF]
, 2013 Transcription of genes is the focus of most forms of regulation of gene expression. Even though careful biochemical experimentation has revealed the molecular mechanisms of transcription initiation for a number of different promoters in vitro, the dynamics of this process in cells is still poorly understood.
arxiv +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1992 Synthetic pre-edited messenger RNA (mRNA) and guide RNA (gRNA) for the 5'-edited maxicircle-encoded ND7 cryptogene from Leishmania tarentolae formed chimeric molecules upon incubation in the presence of a mitochondrial extract.
B. Blum, L. Simpson
semanticscholar +1 more source
Guardian of Genetic Messenger-RNA-Binding Proteins
Biomolecules, 2016 RNA in cells is always associated with RNA-binding proteins that regulate all aspects of RNA metabolism including RNA splicing, export from the nucleus, RNA localization, mRNA turn-over as well as translation. Given their diverse functions, cells express
Antje Anji, Meena Kumari
doaj +1 more source
Applications and challenges of biomaterial mediated mRNA delivery
Exploration of Targeted Anti-tumor Therapy, 2022 With the rapid development of gene therapy technology and the outbreak of coronavirus disease 2019 (COVID-19), messenger RNA (mRNA) therapeutics have attracted more and more attention, and the COVID-19 mRNA vaccine has been approved by the Food and Drug ...
Huapan Fang, Qian Chen
doaj +1 more source
Systematic identification of abundant A-to-I editing sites in the human transcriptome [PDF]
Nature Biotechnology 22, 1001-1005 (2004), 2004 RNA editing by members of the double-stranded RNA-specific ADAR family leads to site-specific conversion of adenosine to inosine (A-to-I) in precursor messenger RNAs. Editing by ADARs is believed to occur in all metazoa, and is essential for mammalian development.
arxiv +1 more source
Defective control of pre–messenger RNA splicing in human disease
Journal of Cell Biology, 2016 Examples of associations between human disease and defects in pre–messenger RNA splicing/alternative splicing are accumulating. Although many alterations are caused by mutations in splicing signals or regulatory sequence elements, recent studies have ...
B. Chabot, L. Shkreta
semanticscholar +1 more source