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Isolation of plant mitochondrial RNA
1986Publisher Summary This chapter discusses the methods for the isolation of plant mitochondrial RNA (mt RNA). The analysis of plant mitochondrial transcripts necessitates the preparation of RNA isolated from mitochondria rather than total RNA. The procedures required to separate plant mitochondria from other subcellular components are time consuming ...
D B, Stern, K J, Newton
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Molecular Biology Reports, 1990
We have recently shown that although pre-mRNA splicing in plants shares some features in common with splicing in vertebrates, there are some crucial differences. In plants there is a requirement for a general enrichment for A+U within the intron and there is no requirement for a 3 ' polypyrimidine tract (Goodall and Filipowicz, 1989).
Goodall G +3 more
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We have recently shown that although pre-mRNA splicing in plants shares some features in common with splicing in vertebrates, there are some crucial differences. In plants there is a requirement for a general enrichment for A+U within the intron and there is no requirement for a 3 ' polypyrimidine tract (Goodall and Filipowicz, 1989).
Goodall G +3 more
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RNA metabolism in plant mitochondria
Trends in Plant Science, 2014Mitochondria are essential for the eukaryotic cell and are derived from the endosymbiosis of an α-proteobacterial ancestor. Compared to other eukaryotes, RNA metabolism in plant mitochondria is complex and combines bacterial-like traits with novel features that evolved in the host cell.
Kamel, Hammani, Philippe, Giegé
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RNA Editing in Plant Mitochondria
Science, 1989Comparative sequence analysis of genomic and complementary DNA clones from several mitochondrial genes in the higher plant Oenothera revealed nucleotide sequence divergences between the genomic and the messenger RNA-derived sequences.
R, Hiesel +3 more
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Advances in imaging RNA in plants
Trends in Plant Science, 2010Increasing evidence shows that many RNAs are targeted to specific locations within cells, and that RNA-processing pathways occur in association with specific subcellular structures. Compartmentation of mRNA translation and RNA processing helps to assemble large RNA-protein complexes, while RNA targeting allows local protein synthesis and the asymmetric
Nynne M, Christensen +2 more
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Regulatory small RNAs in plants
2007The discovery of microRNAs in the last decade altered the paradigm that protein coding genes are the only significant components for the regulation of gene networks. Within a short period of time small RNA systems within regulatory networks of eukaryotic cells have been uncovered that will ultimately change the way we infer gene regulation networks ...
Cameron, Johnson, Venkatesan, Sundaresan
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Poly(A)-associated RNA in plants
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974The RNA associated with poly(A) sequences from Euglena gracilis and Vicia faba has been isolated by binding to millipore filters and characterized by sedimentation velocity centrifugation and electrophoretic mobility. Poly(A)-associated RNA as isolated in solution was highly aggregated. When denatured, it sedimented as a broad peak with a mean value of
D, Sagher, M, Edelman, K M, Jakob
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Messenger RNA Modifications in Plants
Trends in Plant Science, 2019Over 160 distinct RNA modifications are known and collectively termed the epitranscriptome. Some of these modifications have been discovered in mRNA, uncovering a new layer of gene regulation. Transcriptome-wide mapping of epitranscriptomic codes and the discovery of their writers, erasers, and readers that dynamically install, remove, and interpret ...
Lisha, Shen +3 more
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Ribosomal RNA synthesis in plants
Journal of Molecular Biology, 1970Abstract The cytoplasmic ribosomal RNA's (of molecular weight 1.3 × 106 and 0.7 × 106 daltons) of higher plants arise by the selective cleavage of larger precursor molecules. The main precursor RNA species in carrot (Daucus carota) have been tentatively characterized by polyacrylamide-gel electrophoresis as having molecular weights of 2.8 × 106, 2.2 ×
C J, Leaver, J L, Key
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Plant Mitochondrial RNA Editing
Journal of Molecular Evolution, 1999RNA editing affects messenger RNAs and transfer RNAs in plant mitochondria by site-specific exchange of cytidine and uridine bases in both seed and nonseed plants. Distribution of the phenomenon among bryophytes has been unclear since RNA editing has been detected in some but not all liverworts and mosses.
S, Steinhauser +4 more
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