Results 261 to 270 of about 126,755 (277)

Scanning tunnelling microscopy of 16S ribosomal RNA in water

Biochemical and Biophysical Research Communications, 1991
The scanning tunnelling microscope has been used to image 16S ribosomal RNA molecules in water electrophoretically deposited on graphite surface. Two kinds of images have been obtained: images showing aggregates of 16S ribosomal RNA molecules similar to those obtained from DNA solutions and others showing individual 16S ribosomal RNA molecules.
E, Lesniewska, P J, Flamion, C, Cachia, J P, Schreiber, J P, Goudonnet   +4 more
openaire   +2 more sources

Interaction of antibiotics with functional sites in 16S ribosomal RNA

Nature, 1987
Chemical footprinting shows that several classes of antibiotics (streptomycin, tetracycline, spectinomycin, edeine, hygromycin and the neomycins) protect concise sets of highly conserved nucleotides in 16S ribosomal RNA when bound to ribosomes. These findings have strong implications for the mechanism of action of these antibiotics and for the ...
D, Moazed, H F, Noller
openaire   +2 more sources

To Knot or Not to Knot? Examination of 16S Ribosomal RNA Models

Journal of Biomolecular Structure and Dynamics, 1998
The presence of topological knots in large RNA structures is highly unlikely given that 1) no RNA structures determined thus far contain topological knots, 2) secondary structure maps for most RNA molecules are knot free, 3) there are no known RNA topoisomerases, and 4) it is difficult to imagine how knots could be formed specifically and uniquely ...
M S, VanLoock, B A, Harris, S C, Harvey
openaire   +2 more sources

Model for the three-dimensional folding of 16 S ribosomal RNA

Journal of Molecular Biology, 1988
We have derived a model for the three-dimensional folding of Escherichia coli 16 S ribosomal RNA, using interactive computer graphic methods. It is based on (1) the secondary structure derived from comparative sequence analysis, (2) the three-dimensional co-ordinates for the centers of mass of the 30 S subunit proteins, and (3) the locations of sites ...
S, Stern, B, Weiser, H F, Noller
openaire   +2 more sources

Complementary binding of oligonucleotides with 16S RNA and ribosomal ribonucleoproteins

Molecular Biology Reports, 1975
The accessibility of single-stranded sequences in 16S RNA in free state and in ribonucleoprotein particles (RNP) to complementary binding with isoplith fractions of oligonucleotides was studied. RNP had different protein composition and corresponded to intermediate stages of E. coli 30S subunit assembly in vitro.
A M, Kopylov, N V, Chichkova, A A, Boganov, S K, Vasilenko   +3 more
openaire   +2 more sources

Methylation of 16S RNA during ribosome assembly in vitro

Nature, 1974
Methyl deficient 16S RNA from kasugamycin resistant Escherichia coli has been used to study methylation of RNA during ribosome assembly in vitro. Methylation occurs at an intermediate stage and is inhibited at a late stage of assembly. 30S ribosomal proteins required for methylation, and inhibition, have been identified.
P, Thammana, W A, Held
openaire   +2 more sources

Images of 16S ribosomal RNA by scanning tunnelling microscopy

Journal of Microscopy, 1991
SUMMARYWe report the use of scanning tunnelling microscopy (STM) to study surface topographies of complex nucleic acid structures. From low‐resolution STM images of uncoated 16S ribosomal RNA, we demonstrate the possibility of determining several objective parameters (molecular mass and radius of gyration) in order to characterize and identify the ...
P J, Flamion, C, Cachia, J P, Schreiber, T, David, E, Lesniewska, J P, Goudonnet   +5 more
openaire   +2 more sources

Structure of synthetic unmethylated 16S Ribosomal RNA as purified RNA and in reconstituted 30S ribosomal subunits

Biochemistry, 1989
16S ribosomal RNA was made by in vitro transcription of a cloned gene, and its structure was compared to authentic 16S ribosomal RNA. The comparison was made by subjecting the two types of 16S rRNA to chemical reagents that react specifically with unpaired bases and determining the extent of reaction by reverse transcription and gel electrophoresis of ...
G, Ericson, K, Chevli, P, Wollenzien
openaire   +2 more sources

[11] 16 S ribosomal RNA cataloging

1988
Publisher Summary This chapter discusses 16 S ribosomal RNA cataloging. Phylogenetic studies on cyanobacteria and chloroplasts using the 16 S ribosomal RNA (rRNA) cataloging approach as a tool have been done by two different methods: the traditional one which is generally not used anymore and the advanced technique which is presented in detail in ...
Erko Stackebrandt, Wolfgang Ludwig
openaire   +1 more source

Small-Angle X-Ray Studies on the Structure of 16-S Ribosomal RNA and of a Complex of Ribosomal Protein S4 and 16-S Ribosomal RNA from Escherichia coli

European Journal of Biochemistry, 1975
16-S ribosomal RNA and a complex of ribosomal protein S4 and 16-S rRNA were studied in solution by small-angle X-ray scattering. Concentration series of the 16-S rRNA and the S4 - 16-S-rRNA complex were measured in 37.5 mM Tris-HCl buffer pH 7.4 at 5 degrees C. The following data were determined.
W, Folkhard, I, Pilz, O, Kratky, R, Garrett, G, Stöffler   +4 more
openaire   +2 more sources