Effective Alu repeat based RT-qPCR normalization in cancer cell perturbation experiments [PDF]
, 2013 Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories.
Beckers, Anneleen +13 more
core +8 more sources
Assessment of 19 Genes and Validation of CRM Gene Panel for Quantitative Transcriptional Analysis of Molecular Rejection and Inflammation in Archival Kidney Transplant Biopsies. [PDF]
, 2019 Background: There is an urgent need to develop and implement low cost, high-throughput standardized methods for routine molecular assessment of transplant biopsies.
Dobi, Dejan +7 more
core
The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]
, 1992 The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert +3 more
core +1 more source
Advanced Healthcare Materials, EarlyView.We measure the cell‐specific responses of administering infusible ECM (iECM) in acute myocardial infarction (MI) across multiple timepoints. Using single‐nucleus RNA sequencing and spatial transcriptomics, we measure macrophage activation, fibroblast remodeling, increased vascular development, lymphangiogenesis, cardioprotection, and neurogenesis ...
Joshua M. Mesfin +18 more
wiley +1 more source
Molecular Characterization of a Non–Babesia divergens Organism Causing Zoonotic Babesiosis in Europe
Emerging Infectious Diseases, 2003 In Europe, most reported human cases of babesiosis have been attributed, without strong molecular evidence, to infection with the bovine parasite Babesia divergens.
Barbara L. Herwaldt +13 more
doaj +1 more source
Cyclic loading of tendon fascicles using a novel fatigue loading system increases interleukin-6 expression by tenocytes [PDF]
, 2013 Repetitive strain or ‘overuse’ is thought to be a major factor contributing to the development of tendinopathy. The aims of our study were to develop a novel cyclic loading system, and use it to investigate the effect of defined loading conditions on the
Jones, Gavin +3 more
core +1 more source
Advanced Healthcare Materials, EarlyView.3D printed hybrid scaffolds combining bioactive silica–calcium chemistry with elastic polymers guide human bone stem cells to form bone. The scaffolds support cell survival, organization, and invasion while releasing osteogenic ions. Together, architecture and composition drive bone‐specific gene expression, extracellular matrix organization, and ...
David R. Sory +3 more
wiley +1 more source
Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]
Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus +50 more
core +1 more source
First documented record of a living solemyid bivalve in a pockmark of the Nile Deep-sea Fan (eastern Mediterranean Sea) [PDF]
, 2011 A living specimen of a solemyid bivalve was collected at bathyal depths near a pockmark in the Nile Deep-sea Fan (eastern Mediterranean) and is here presented.
Clara F. Rodrigues +7 more
core +2 more sources
Sequence determination of the 3' terminal T1 oligonucleotide of 18S ribosomal RNA
Nucleic Acids Research, 1976 We have reexamined the primary structure of the 3' terminal oligonucleotide of 18S RNA from chicken fibroblasts and have shown, contrary to previously published results that this extremity G-A-U-C-A-U-U-AOH is identical to that of the rabbit, drosophila and bombyx.
M E, Eladari, F, Galibert
openaire +3 more sources