Results 141 to 150 of about 138,758 (334)

Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress

BMC Genomics
Background Quantitative Real-time PCR (qRT-PCR) is a powerful technique to analyze gene expression patterns by measuring the relative abundance of mRNA transcription levels.
Ping Han, Jianming Chen, Zhennan Sun, Shengjie Ren, Xubo Wang   +4 more
doaj   +1 more source

Phenotypic plasticity in the Caribbean sponge Callyspongia vaginalis (Porifera: Haplosclerida)

Scientia Marina, 2010
Sponge morphological plasticity has been a long-standing source of taxonomic difficulty. In the Caribbean, several morphotypes of the sponge Callyspongia vaginalis have been observed.
Susanna López-Legentil, Patrick M. Erwin, Timothy P. Henkel, Tse-Lynn Loh, Joseph R. Pawlik   +4 more
doaj   +1 more source

Redefining Therapies for Drug‐Resistant Tuberculosis: Synergistic Effects of Antimicrobial Peptides, Nanotechnology, and Computational Design

Advanced Healthcare Materials, EarlyView.
Antimicrobial peptide (AMP)‐loaded nanocarriers provide a multifunctional strategy to combat drug‐resistant Mycobacterium tuberculosis. By enhancing intracellular delivery, bypassing efflux pumps, and disrupting bacterial membranes, this platform restores phagolysosome fusion and macrophage function.
Christian S. Carnero Canales, Jessica Ingrid Marquez Cazorla, Renzo Marianito Marquez Cazorla, Aline Martins dos Santos, Jonatas Lobato Duarte, Letícia Oliveira Catarin Nunes, Túlio Custódio Reis, Lara Cerazi Salvador, Norival Alves Santos‐Filho, Rafael Miguel Sábio, Hélder A. Santos, Fernando Rogério Pavan   +11 more
wiley   +1 more source

The pathway of hepatitis C virus mRNA recruitment to the human ribosome. [PDF]

, 2009
Eukaryotic protein synthesis begins with mRNA positioning in the ribosomal decoding channel in a process typically controlled by translation-initiation factors.
Doudna, Jennifer A, Fraser, Christopher S, Hershey, John WB   +2 more
core  

HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation. [PDF]

, 2013
The internal ribosome entry site (IRES) of the hepatitis C virus (HCV) drives noncanonical initiation of protein synthesis necessary for viral replication.
A Ben-Shem, A Devaraj, AA Malygin, AD Petropoulos, AY Keel, Breanna S Vollmar, C Chen, CM Spahn, CS Fraser, CS Fraser, D Boehringer, D Hartz, D Sousa, Dan Shi, DD Ian'shina, DV Sizova, EF Pettersen, F Odreman-Macchioli, F Peske, F Robert, GA Otto, GJ Kleywegt, H Ji, IB Lomakin, IM Terenin, J Bukh, J Rabl, J Wower, JA Mindell, JE Wilson, Jeffrey S Kieft, JH Kim, JM Fringer, JR Lytle, JR Lytle, JS Kieft, JS Kieft, K Tsukiyama-Kohara, KE Berry, KI Kalliampakou, LA Passmore, M Honda, M Ohi, M Selmer, M Stoneley, M van Heel, ME Filbin, Megan E Filbin, MG Acker, MM Yusupov, N Grigorieff, N Locker, O Galkin, P Antúnez de Mayolo, P Simmonds, PJ Lukavsky, PJ Lukavsky, RE Monro, RJ Jackson, S Fukushi, S Uemura, SM Dibrov, T Döring, Tamir Gonen, TV Pestova, TV Pestova, U Geigenmüller, VG Kolupaeva, Y Yu   +68 more
core   +1 more source

Mesenchymal Stem Cell‐Derived Apoptotic Micro‐Vesicles Repaired Sciatic Nerve Defect by Regulating Early Inflammatory Microenvironment and Promoting Angiogenesis

Advanced Healthcare Materials, EarlyView.
HUCMSC‐Apo‐mvs enhance peripheral nerve repair by modulating the inflammatory microenvironment (IME), primarily through coordinated actions on three functional cells. They recruit macrophages and promote their polarization from pro‐inflammatory M1 to anti‐inflammatory M2 phenotypes, increasing secretion of IL‐10 and VEGF.
Haolin Liu, Yiben Ouyang, Bo Wang, Xiaochun Zhang, Yanjun Guan, Ruichao He, Yuhui Cui, Junli Wang, Qianru Yao, Yixiao Tan, Xiwei Peng, Xing Xiong, Sice Wang, Dongdong Li, Haofeng Cheng, Tianqi Su, Xiaoyang Fu, Jinjuan Zhao, Mingqian Yu, Yi Wang, Jiang Peng, Yu Wang   +21 more
wiley   +1 more source

A 3D Astrocyte Microenvironment Model Enables Rapid Ca2+‐Resolved Analysis and Therapeutic Modulation of Oxidative Neuroinflammation

Advanced Healthcare Materials, EarlyView.
This work presents ARC‐3D, a soft 3D model that recreates how brain support cells, called astrocytes, react to oxidative stress. The system visualizes rapid calcium changes and inflammatory signals, and shows how the drug KDS12025 can protect cells from damage. ARC‐3D offers a simple, reliable way to study early drivers of brain inflammation.
Ju‐Kang Kim, Ye‐Ji Kim, Seyoung Ryu, Daeui Park, Kyoung‐Sik Moon, Yu Bin Lee, Dong Ho Woo   +6 more
wiley   +1 more source

siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA

Advanced Healthcare Materials, EarlyView.
Osteogenic‐angiogenic cross‐talk is a vital prerequisite for vascularized bone regeneration. In this study, we investigated the effects of siRNA‐mediated silencing of two inhibitory proteins, Chordin and WWP‐1, via CaP‐NP‐loaded gelatin microparticles in osteogenically differentiated microtissues.
Franziska Mitrach, Jonas Kubat, Stefan Simm, Alexandra H. Springwald, Burak Demir, Anton Liebezeit, Michael C. Hacker, Michaela Schulz‐Siegmund   +7 more
wiley   +1 more source

Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation.

PLoS Genetics, 2017
Box C/D snoRNAs are known to guide site-specific ribose methylation of ribosomal RNA. Here, we demonstrate a novel and unexpected role for box C/D snoRNAs in guiding 18S rRNA acetylation in yeast.
Sunny Sharma, Jun Yang, Rob van Nues, Peter Watzinger, Peter Kötter, Denis L J Lafontaine, Sander Granneman, Karl-Dieter Entian   +7 more
doaj   +1 more source

Secondary structures in polyoma DNA [PDF]

, 1979
Three reproducible secondary-structure features were observed on single strands of polyoma virus DNA mounted for electron microscopy by the T4 gene 32 protein technique: (i) a hairpin fold-back extending from 92.9 +/- 0.8 to 95.0 +/- 0.7 map units; (ii ...
Davidson, Norman, Manor, Haim, Wu, Madeline   +2 more
core