Results 11 to 20 of about 587,009 (267)

Structural and functional analysis of Utp24, an endonuclease for processing 18S ribosomal RNA. [PDF]

open access: yesPLoS ONE, 2018
The precursor ribosomal RNA is processed by multiple steps of nucleolytic cleavage to generate mature rRNAs. Utp24 is a PIN domain endonuclease in the early 90S precursor of small ribosomal subunit and is proposed to cleave at sites A1 and A2 of pre-rRNA.
Weidong An, Yifei Du, Keqiong Ye
doaj   +4 more sources

Optimal eukaryotic 18S and universal 16S/18S ribosomal RNA primers and their application in a study of symbiosis.

open access: yesPLoS ONE, 2014
Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems.
Yong Wang   +4 more
doaj   +5 more sources

18S Ribosomal RNA and Tetrapod Phylogeny [PDF]

open access: yesSystematic Biology, 2003
Previous phylogenetic analyses of tetrapod 18S ribosomal RNA (rRNA) sequences support the grouping of birds with mammals, whereas other molecular data, and morphological and paleontological data favor the grouping of birds with crocodiles. The 18S rRNA gene has consequently been considered odd, serving as "definitive evidence of different genes ...
Karl M. Kjer   +3 more
openaire   +3 more sources

Structure of 18 S Ribosomal RNA in Native 40 S Ribosomal Subunits [PDF]

open access: yesJournal of Biological Chemistry, 1997
We have analyzed the structure of 18 S rRNA in native 40 S subunits using chemical modification followed by primer extension. The native subunits were modified using the single-stranded specific reagents dimethyl sulfate and 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate.
Lovisa Holmberg   +2 more
openaire   +3 more sources

Basepairing with 18S ribosomal RNA in internal initiation of translation [PDF]

open access: yesFEBS Letters, 1994
In concert with the translation initiation factors ‘trans‐acting’ factors function specifically during internal initiation on picornaviral mRNAs. Of these trans‐acting factors, two have been identified as the La‐protein and the polypyrimidine tract binding protein.
Gert C. Scheper   +2 more
openaire   +4 more sources

The terminal sequences of Bombyx mori 18S ribosomal RNA [PDF]

open access: yesNucleic Acids Research, 1975
The 5' and 3' terminal T1 oligonucleotides of 32p-labelled B. mori 18S ribosomal RNA were isolated by a two dimensional electrophoretic (diagonal) technique. Nucleotide sequence analysis showed that the 3' terminal fragment, (G)AUCAUUAOH, is identical to that previously obtained from the 18S rRNA of several other eukaryotic species. The sequence of the
Richard A. Kramer   +2 more
openaire   +3 more sources

Arabidopsis RIBOSOMAL RNA PROCESSING7 Is Required for 18S rRNA Maturation [PDF]

open access: yesThe Plant Cell, 2018
Ribosome biogenesis is fundamental to growth and development in eukaryotes and is linked to human diseases and cancer. Arabidopsis thaliana MORPHOLOGY OF ARGONAUTE1-52 SUPPRESSED 2 (MAS2) participates in splicing and 45S ribosomal DNA (rDNA) expression.
Rosa Micol-Ponce   +5 more
openaire   +4 more sources

Identification of stable reference genes for quantitative gene expression analysis in the duodenum of meat-type ducks

open access: yesFrontiers in Veterinary Science, 2023
Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui   +13 more
doaj   +1 more source

18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA [PDF]

open access: yesBioMed Research International, 2016
The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis.
Cory Ann Leonard   +3 more
openaire   +3 more sources

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