18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA [PDF]
BioMed Research International, 2016 The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis.
Cory Ann Leonard +3 more
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The terminal sequences of Bombyx mori 18S ribosomal RNA
Nucleic Acids Research, 1975 The 5' and 3' terminal T1 oligonucleotides of 32p-labelled B. mori 18S ribosomal RNA were isolated by a two dimensional electrophoretic (diagonal) technique. Nucleotide sequence analysis showed that the 3' terminal fragment, (G)AUCAUUAOH, is identical to that previously obtained from the 18S rRNA of several other eukaryotic species. The sequence of the
K U, Sprague, R A, Kramer, M B, Jackson
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Structure of 18 S Ribosomal RNA in Native 40 S Ribosomal Subunits [PDF]
Journal of Biological Chemistry, 1997 We have analyzed the structure of 18 S rRNA in native 40 S subunits using chemical modification followed by primer extension. The native subunits were modified using the single-stranded specific reagents dimethyl sulfate and 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate.
Y, Melander, L, Holmberg, O, Nygârd
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Stably expressed housekeeping genes across developmental stages in the two-spotted spider mite, Tetranychus urticae. [PDF]
PLoS ONE, 2015 Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for measuring mRNA expression. To facilitate gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to stable housekeeping genes is mandatory.
Chunxiao Yang +3 more
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RNA Biology, 2022 snR30/U17 is a highly conserved H/ACA RNA that is required for maturation of the small ribosomal subunit in eukaryotes. By base-pairing to the expansion segment 6 (ES6) of 18S ribosomal RNA (rRNA), the snR30 H/ACA Ribonucleoprotein (RNP) indirectly ...
Timothy J. Vos, Ute Kothe
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Arabidopsis RIBOSOMAL RNA PROCESSING7 Is Required for 18S rRNA Maturation [PDF]
The Plant Cell, 2018 Ribosome biogenesis is fundamental to growth and development in eukaryotes and is linked to human diseases and cancer. Arabidopsis thaliana MORPHOLOGY OF ARGONAUTE1-52 SUPPRESSED 2 (MAS2) participates in splicing and 45S ribosomal DNA (rDNA) expression.
Rosa Micol-Ponce +5 more
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RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components [PDF]
, 2004 Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors to the small subunit RNA, the 18S, of the ribosome. We
Granneman, Sander; id_orcid +6 more
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The putative NTPase Fap7 mediates cytoplasmic 20S pre-rRNA processing through a direct interaction with Rps14 [PDF]
, 2005 One of the proteins identified as being involved in ribosome biogenesis by high-throughput studies, a putative P-loop-type kinase termed Fap7 (YDL166c), was shown to be required for the conversion of 20S pre-rRNA to 18S rRNA.
Nandineni, Madhusudan R +2 more
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Recent Advances on the Structure and Function of RNA Acetyltransferase Kre33/NAT10
Cells, 2019 Ribosome biogenesis is one of the most energy demanding processes in the cell. In eukaryotes, the main steps of this process occur in the nucleolus and include pre-ribosomal RNA (pre-rRNA) processing, post-transcriptional modifications, and assembly of ...
Sophie Sleiman, Francois Dragon
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Karyotypic analysis and isolation of four DNA markers of the scleractinian coral Favites pentagona (Esper, 1795) (Scleractinia, Anthozoa, Cnidaria) [PDF]
Comparative Cytogenetics, 2022 We performed conventional and molecular cytogenetic studies on the Favites pentagona Esper, 1795, a scleractinian coral mostly found along the west coast of Japan. Karyotype analysis of F.
Rei Kawakami +6 more
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