Results 191 to 200 of about 484,088 (306)

Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is a widely used delivery vehicle in gene therapy. A scalable production technology is essential for its wide clinical applications. We have taken a synthetic biology approach to generate HEK293‐based cell lines which harbor integrated genetic elements encoding essential AAV and adenoviral helper ...
Han‐Jung Kuo, Prahalad Srinivasan, Yu‐Chieh Lin, Min Lu, Carissa Rungkittikhun, Qi Zhang, Wei‐Shou Hu   +6 more
wiley   +1 more source

Gene Expression Depends on the Interplay Among Growth, Resource Biogenesis, and Nutrient Quality. [PDF]

ACS Synth Biol
Kim J, Darlington APS, Muñoz-Montero S, Montenegro R, Dalby P, Herrera-Martín N, Banks A, Prakash S, Polizzi K, Bates DG, Jiménez JI.   +10 more
europepmc   +1 more source

N6‐methyladenosine‐regulated exosome biogenesis orchestrates an immunosuppressive pre‐metastatic niche in gastric cancer peritoneal metastasis

Cancer Communications, EarlyView.
Abstract Background Gastric cancer peritoneal metastasis is clinically challenging, given the limited treatment options and poor prognosis. The molecular mechanisms that precede gastric cancer peritoneal metastasis, known as the pre‐metastatic niche (PMN), and its relationship with N6‐methyladenosine (m6A) modification remain unclear.
Song Li, Jianyuan Zhou, Shuang Wang, Qian Yang, Shulun Nie, Chunwang Ji, Xue Zhang, Shuhan Li, Xuanyu Zhou, Jiahui Chu, Xuehui Wu, Jianqiao Jiao, Ruitao Xu, Qian Xu, Miao Huang, Qiushi Wang, Liliang Dou, Qinqin Hu, Fan Jiang, Xin Dai, Zhaodi Nan, Xinyu Song, Di Zhang, Lian Liu   +23 more
wiley   +1 more source

Multi-omics analysis reveals CMTR1 upregulation in cancer and roles in ribosomal protein gene expression and tumor growth. [PDF]

Cell Commun Signal
Campeanu IJ, Jiang Y, Afisllari H, Dzinic S, Polin L, Yang ZQ.   +5 more
europepmc   +1 more source

Piper auritum (Kunth) Fungal Endophytes: A Source of Metabolites to Inhibit the Formation of Advanced Glycation End Products

Chemistry &Biodiversity, EarlyView.
Endophytic fungi of P. auritum were evaluated for their ability to produce inhibitors of formation of advanced glycation end products (AGEs). Neopestalotiopsis sp. H2 and Diaporthe sp. H5 extracts were the most active. So, we performed their chemical profiling by untargeted and phenolics‐targeted metabolomic approaches to identify the potential ...
Lory Sthephany Rochín‐Hernández, José Antonio Guerrero‐Analco, Juan Luis Monribot‐Villanueva, Luis Bernardo Flores‐Cotera   +3 more
wiley   +1 more source

Poly(ethylene glycol)‐based Peptidomimetics (Pegtides) of Antimicrobial Peptides

ChemBioChem, Accepted Article.
Cationic amphipathic poly(ethylene glycol) (PEG)‐based polymers were generated with synthetic efficiencies allowing their evaluation as Antimicrobial Peptide (AMP) mimetics. Accordingly, statistical copolymers with cationic units consistently functionalised with guanyl groups, but different side‐chain lengths, and hydrophobic units displaying long ...
Conor Shine, John R. F. B. Connolly, Robert D. Murphy, Hazel Lafferty, Abdalmalek Alfnikh, Ned P. Buijs, Hawraa Shahrour, Nathaniel I. Martin, Eoghan O'Neill, George Amarandei, Jimmy Muldoon, Marc Maresca, Deirdre Fitzgerald-Hughes, Marc Devocelle   +13 more
wiley   +1 more source

The Central Role of Ribosomal Proteins in p53 Regulation. [PDF]

Cancers (Basel)
Lindström MS.
europepmc   +1 more source

Mammalian Tolerance to Amino Acid Heterochirality

ChemBioChem, EarlyView.
Organisms preferentially use l‐amino acids (l‐AAs). However, de novo synthesized l‐AAs are in part converted to d‐AAs. The ribosome has a robust system to use l‐AAs for protein synthesis, whereas non‐ribosomal synthesis can also use d‐AAs. Furthermore, some AA residues can be post‐translationally isomerized through enzymatic or spontaneous processes ...
Sakiko Taniguchi, Kenichiro Adachi, Xuan Tran, Masataka Suzuki, Jumpei Sasabe   +4 more
wiley   +1 more source

mTORC1 cooperates with tRNA wobble modification to sustain the protein synthesis machinery. [PDF]

Nat Commun
Hermann J, Borteçen T, Kalis R, Kowar A, Pechincha C, Vogt V, Schneider M, Helm D, Krijgsveld J, Loayza-Puch F, Zuber J, Palm W.   +11 more
europepmc   +1 more source

Peptidomimetics Targeting the Amyloidogenicity of Nucleophosmin 1 Mutations in Acute Myeloid Leukemia

ChemBioChem, EarlyView.
Previous studies reveal an unexpected tendency for amyloid aggregation in specific regions of the C‐terminal domain (CTD) of nucleophosmin 1 (NPM1), a nucleolar chaperone involved in acute myeloid leukemia (AML). Herein, several peptide analogs of NPM1264‐277 (amyloid core) with conservative substitutions are designed and evaluated through biophysical ...
Daniele Florio, Sara La Manna, Giada Raffaella Fiore, Valentina Roviello, Giuliano Castellano, Sossio Fabio Graziano, Anna Maria Malfitano, Daniela Marasco   +7 more
wiley   +1 more source