MINTmap: fast and exhaustive profiling of nuclear and mitochondrial tRNA fragments from short RNA-seq data. [PDF]
Transfer RNA fragments (tRFs) are an established class of constitutive regulatory molecules that arise from precursor and mature tRNAs. RNA deep sequencing (RNA-seq) has greatly facilitated the study of tRFs.
Loher, Phillipe+2 more
core +2 more sources
Next generation sequencing reveals the complete plastome sequence of newly discovered cliff-dwelling Sonchus boulosii (Asteraceae: Cichorieae) in Morocco [PDF]
The complete chloroplast genome sequences of newly discovered cliff-dwelling species of Sonchus, S. boulosii, were reported in this study. The S. boulosii plastome was 152,016 bp long, with the large single copy (LSC) region of 83,988 bp, the small ...
Kim, Seon-Hee+2 more
core +1 more source
Discoba (Excavata) is an ancient group of eukaryotes with great morphological and ecological diversity. Unlike the other major divisions of Discoba (Jakobida and Euglenozoa), little is known about the mitochondrial DNAs(mtDNAs) of Heterolobosea.
Andersson, Siv G. E.+4 more
core +2 more sources
ER Membrane Contact Sites: Key Platforms for Biogenesis of RNA-Containing Extracellular Vesicles
The mechanisms by which cytoplasmic cargoes such as RNAs are incorporated into extracellular vesicles (EVs) are poorly understood. In a recent article published in Developmental Cell, we describe a novel function of endoplasmic reticulum membrane contact
Bahnisikha Barman+2 more
doaj +1 more source
Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA
A simple and fast RNA gel blot procedureis described that uses 50 mM NaOH tosimultaneously transfer and fix RNA to apositively charged nylon membrane. TheRNA is transferred in a downward direction,and transfer is routinely completedwithin 2.5 h.
Ivan L. Ingelbrecht+2 more
doaj +1 more source
The complete mitogenome sequence of Sinogastromyzon szechuanensis was obtained using next generation sequencing and confirmed via overlap-PCR method.
Wei Li+7 more
doaj +1 more source
Small RNAs and extracellular vesicles in filarial nematodes: from nematode development to diagnostics [PDF]
Parasitic nematodes have evolved sophisticated mechanisms to communicate with their hosts in order to survive and successfully establish an infection. The transfer of RNA within extracellular vesicles (EVs) has recently been described as a mechanism that
Babayan, S.A.+2 more
core +2 more sources
Characterization of UVA-Induced Alterations to Transfer RNA Sequences
Ultraviolet radiation (UVR) adversely affects the integrity of DNA, RNA, and their nucleoside modifications. By employing liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based RNA modification mapping approaches, we identified the transfer RNA (
Congliang Sun+2 more
doaj +1 more source
BRUCE: a program for the detection of transfer-messenger RNA genes in nucleotide sequences [PDF]
A computer program, BRUCE, was developed for the identification of transfer‐messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNAAla‐like secondary structure surrounding a short sequence encoding the tag peptide.
Andersson, S., Canback, B., Laslett, D.
core +3 more sources
YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs. [PDF]
Besides translation, transfer RNAs (tRNAs) play many non-canonical roles in various biological pathways and exhibit highly variable expression profiles.
Honda, Shozo+5 more
core +2 more sources