Results 21 to 30 of about 925,090 (301)

A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA

open access: yesNature Communications, 2020
Extracellular vesicles (EV) facilitate intercellular transfer of biological material including RNA, but the regulatory mechanisms for their formation and transfer are incompletely known.
Olivier G. de Jong   +13 more
doaj   +1 more source

Structural insights into RNA processing by the human RISC-loading complex. [PDF]

open access: yes, 2009
Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC).
AD Haase   +41 more
core   +2 more sources

Multiple effects of silymarin on the hepatitis C virus lifecycle [PDF]

open access: yes, 2010
Silymarin, an extract from milk thistle (Silybum marianum), and its purified flavonolignans have been recently shown to inhibit hepatitis C virus (HCV) infection, both in vitro and in vivo.
Bourne, Nigel   +16 more
core   +1 more source

ER Membrane Contact Sites: Key Platforms for Biogenesis of RNA-Containing Extracellular Vesicles

open access: yesContact, 2022
The mechanisms by which cytoplasmic cargoes such as RNAs are incorporated into extracellular vesicles (EVs) are poorly understood. In a recent article published in Developmental Cell, we describe a novel function of endoplasmic reticulum membrane contact
Bahnisikha Barman   +2 more
doaj   +1 more source

Sequence organization of feline leukemis virus DNA in infected cells [PDF]

open access: yes, 1980
A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W.   +3 more
core   +1 more source

Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA

open access: yesBioTechniques, 1998
A simple and fast RNA gel blot procedureis described that uses 50 mM NaOH tosimultaneously transfer and fix RNA to apositively charged nylon membrane. TheRNA is transferred in a downward direction,and transfer is routinely completedwithin 2.5 h.
Ivan L. Ingelbrecht   +2 more
doaj   +1 more source

Nanodelivery of a functional membrane receptor to manipulate cellular phenotype. [PDF]

open access: yes, 2018
Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging ...
Baikoghli, Mo   +7 more
core   +2 more sources

Characterization of UVA-Induced Alterations to Transfer RNA Sequences

open access: yesBiomolecules, 2020
Ultraviolet radiation (UVR) adversely affects the integrity of DNA, RNA, and their nucleoside modifications. By employing liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based RNA modification mapping approaches, we identified the transfer RNA (
Congliang Sun   +2 more
doaj   +1 more source

Mitochondrial Translation Products before and after Integration into the Mitochondrial Membrane in Neurospora crassa [PDF]

open access: yes, 1972
# 1. Nascent translation products on mitochondrial ribosomes were selectively labeled in vivo in the presence of cycloheximide with radioactive leucine. They were isolated together with the ribosomes. # 2.
Michel, Rainer, Neupert, Walter
core   +1 more source

Complete mitochondrial genome of Sinogastromyzon szechuanensis (Teleostei, Cypriniformes, Balitoridae) obtained using next-generation sequencing

open access: yesMitochondrial DNA. Part B. Resources, 2021
The complete mitogenome sequence of Sinogastromyzon szechuanensis was obtained using next generation sequencing and confirmed via overlap-PCR method.
Wei Li   +7 more
doaj   +1 more source

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