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Purification of RNA-Binding Proteins

2014
The majority of regulatory RNA sequences exert their function through interaction with proteins. Therefore, the identification of RNA-binding proteins is the key step in understanding the role of many RNA motifs. Here, we describe a straightforward method to identify RNA-binding proteins.
Birgit, Ritter, Marc R, Reboll
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RNA-binding proteins tamed

Nature Structural & Molecular Biology, 1998
Novel RNA-binding proteins with customized specificities can be isolated by genetic selection from combinatorial libraries. Such proteins have great potential as agents for targeted manipulation of gene expression.
Ite A. Laird-Offringa, Joel G. Belasco
openaire   +1 more source

RNA-binding proteins in cellular senescence

Mechanisms of Ageing and Development, 2023
Cellular senescence is a state of irreversible cell cycle arrest that is triggered and controlled by various external and/or internal factors. Among them, the regulation of senescence-associated genes is an important molecular event that plays a role in senescence.
Dahyeon Koh   +4 more
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Single stranded RNA binding proteins

Current Opinion in Structural Biology, 2000
Our knowledge of protein interactions with RNA molecules has been, so far, largely restricted to cases in which the RNA itself is folded into a secondary and/or tertiary structure stabilised by intramolecular base pairing and stacking. Until recently, only limited structural information has been available about protein interactions with single-stranded
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RNA-binding proteins of bovine rotavirus

Journal of Virology, 1986
Two major bovine rotavirus proteins have RNA-binding activity as shown by an RNA overlay-protein blot assay. Of the six proteins in purified virions, only one showed RNA-binding activity. This 92,000-molecular-weight (92K) protein was present in both single- and double-shelled particles.
J F, Boyle, K V, Holmes
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RBM4: A multifunctional RNA-binding protein

The International Journal of Biochemistry & Cell Biology, 2009
RBM4, also known as Lark, was described initially as having a role in circadian rhythm control in Drosophila. In the last 5 years data have emerged from studies of mammalian cells. It is now clear that RBM4 is an RNA-binding protein involved in diverse cellular processes that include alternative splicing of pre-mRNA, translation, and RNA silencing. Its
M Andrea, Markus, Brian J, Morris
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The RNA-binding proteins

2015
This chapter details how RNA-binding proteins package RNA, protect RNA, organize RNA, and prepare RNA for post-transcriptional processes. It describes different kinds of RNA-binding and auxiliary domains that enable RNA-binding proteins to bind RNA in a versatile way.
David Elliott, Michael Ladomery
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RNA binding proteins

2022
Stephen Neidle, Mark Sanderson
openaire   +1 more source

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