Results 121 to 130 of about 524,312 (161)
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RNA-dependent RNA polymerases in RNA silencing
Biological Chemistry, 2011Abstract RNA-dependent RNA polymerases (RdRPs) synthesize double-stranded RNAs that are processed into small RNAs and mediate gene silencing. Viral RdRPs and cellular RdRPs show little structural homology to each other. Cellular RdRPs play key roles in RNA silencing by producing complementary strands for target RNAs via Dicer-dependent and -
Yoshiko, Maida, Kenkichi, Masutomi
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RNA-dependent RNA polymerases from Flaviviridae
Current Opinion in Structural Biology, 2009Viral genome replication in Flaviviridae is carried out by a virally encoded RNA-dependent RNA polymerase (RdRp). These viruses initiate the RNA synthesis via a de novo mechanism that differs from the primer-dependent mechanism used by Picornaviridae. Like all polymerases, the structure of Flaviviridae RdRps resembles a right hand with characteristic ...
Kyung H, Choi, Michael G, Rossmann
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RNA-dependent RNA polymerase in Chinese cabbage
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971Abstract An RNA-dependent RNA polymerase which incorporates nucleotides from ribonucleoside triphosphates into an acid-insoluble polymer has been purified from the chloroplastic fraction of Chinese cabbage leaves. 1. 1. Addition of RNA template is required for enzymatic activity; the synthetic polynucleotide poly (U, C) and TYMV-RNA are among the
S, Astier-Manifacier, P, Cornuet
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L protein, the RNA-dependent RNA polymerase of hantaviruses
Archives of Virology, 2004L protein of hantaviruses is the RNA transcriptase and replicase that transcribes mRNAs and replicates the genomic RNA using antigenomic RNA as an intermediate. It also appears to have endonuclease activity. In this review, the current knowledge on the hantavirus L protein is presented including sequence motifs conserved in RNA polymerases, mechanisms ...
S K J, Kukkonen, A, Vaheri, A, Plyusnin
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RNA-Dependent RNA Polymerases, Viruses, and RNA Silencing
Science, 2002Most viruses have RNA genomes that are replicated and transcribed into messenger RNA by viral RNA–dependent RNA polymerases (RdRps), usually in concert with other viral and host factors. Many, if not most, eukaryotes also encode putative RdRps that have been implicated in sequence-specific, RNA-triggered gene silencing.
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RNA-dependent RNA polymerases from flaviviruses and Picornaviridae
Current Opinion in Structural Biology, 2009Flaviviruses and picornaviruses are positive-strand RNA viruses that encode the RNA-dependent RNA polymerase (RdRp) required for replicating the viral genome in infected cells. Because of their specific and essential role in the virus life cycle, RdRps are prime targets for antiviral drugs.
Julien, Lescar, Bruno, Canard
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An RNA-dependent RNA polymerase is required for paramutation in maize
Nature, 2006Paramutation is an allele-dependent transfer of epigenetic information, which results in the heritable silencing of one allele by another. Paramutation at the b1 locus in maize is mediated by unique tandem repeats that communicate in trans to establish and maintain meiotically heritable transcriptional silencing.
Mary, Alleman +7 more
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Biochemical characterization of rhinovirus RNA-dependent RNA polymerase
Antiviral Research, 2002Human rhinoviruses (HRV) represent the single most important causative agent of the common cold. The HRV genome encodes an RNA-dependent RNA polymerase (RdRp) designated 3D polymerase that is required for replication of the HRV RNA genome. We have expressed and purified recombinant HRV-16 3D polymerase to near homogeneity from Escherichia coli ...
Magdeleine, Hung +2 more
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Viral RNA-Dependent RNA Polymerases: A Structural Overview
2018Most emerging and re-emerging human and animal viral diseases are associated with RNA viruses. All these pathogens, with the exception of retroviruses, encode a specialized enzyme called RNA-dependent RNA polymerase (RdRP), which catalyze phosphodiester-bond formation between ribonucleotides (NTPs) in an RNA template-dependent manner.
Diego, Ferrero +2 more
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Isolation and Analysis of Plant RNA-Dependent RNA Polymerases
2012RNA-dependent RNA polymerases (RDRPs) are encoded by RNA viruses as well as eukaryotic organisms such as plants. The function of these cellular RDRPs has been associated with the synthesis of short interfering RNAs (siRNAs), which are essential regulators of genomic integrity and plant viral defense. The multiple gene copies, and functional diversities,
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