Results 311 to 320 of about 325,869 (333)
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Molecular mechanisms of the RNA polymerases in plant RNA-directed DNA methylation
Trends in Biochemical SciencesIn plants, two atypical DNA-dependent RNA polymerases, RNA polymerase IV (Pol IV) and Pol V, and an RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) together produce noncoding RNAs (ncRNAs) to guide the plant-specific RNA-directed DNA methylation (RdDM). Although both Pol IV and Pol V have evolved from the canonical Pol II, they have adapted to different roles in
Guohui Xie +3 more
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Analytical Biochemistry, 1973
Abstract Analysis of the products of the RNA-directed DNA polymerase reaction using glycerol-substituted polyacrylamide gels has proven superior to the glycerol gradient method for quantitative assay of a series of nucleic acid samples. To overcome the phenomenon of a smearing of background radioactive counts in the gels caused by unincorporated 3H ...
J J, McCormick, L, Calhoun
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Abstract Analysis of the products of the RNA-directed DNA polymerase reaction using glycerol-substituted polyacrylamide gels has proven superior to the glycerol gradient method for quantitative assay of a series of nucleic acid samples. To overcome the phenomenon of a smearing of background radioactive counts in the gels caused by unincorporated 3H ...
J J, McCormick, L, Calhoun
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Journal of Medicinal Chemistry, 1980
Twenty-two 3-substituted rifamycins were tested for inhibition of mammalian alpha and beta DNA polymerase and viral RNA-dependent DNA polymerase ("reverse transcriptase"). Quantitative structure--activity relationships (QSAR) were formulated for the three systems.
R S, Wu +2 more
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Twenty-two 3-substituted rifamycins were tested for inhibition of mammalian alpha and beta DNA polymerase and viral RNA-dependent DNA polymerase ("reverse transcriptase"). Quantitative structure--activity relationships (QSAR) were formulated for the three systems.
R S, Wu +2 more
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Biochemical and Biophysical Research Communications, 1981
Abstract In the presence of optimal concentrations of Mg 2+ , spermine and spermidine were found to stimulate rabbit globin mRNA-directed cDNA synthesis by Rauscher murine leukemia virus (R-MuLV) DNA polymerase. Stimulation of DNA synthesis did not occur with the polyamines putrescine or cadaverine, nor could exogenously provided salt or ammonium ...
S L, Marcus, S W, Smith, C J, Bacchi
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Abstract In the presence of optimal concentrations of Mg 2+ , spermine and spermidine were found to stimulate rabbit globin mRNA-directed cDNA synthesis by Rauscher murine leukemia virus (R-MuLV) DNA polymerase. Stimulation of DNA synthesis did not occur with the polyamines putrescine or cadaverine, nor could exogenously provided salt or ammonium ...
S L, Marcus, S W, Smith, C J, Bacchi
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Journal of Virology, 1975
Reticuloendotheliosis virus (REV) contains an endogenously instructed, RNA-directed DNA polymerase activity. Both the endogenous and exogenous DNA polymerase activities exhibited up to 10-fold greater activity at the optimum concentration of manganous ion (0.025 mM for exogenous; 0.25 mM for endogenous) than at any concentration of magnesium ion ...
M R, Waite, P T, Allen
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Reticuloendotheliosis virus (REV) contains an endogenously instructed, RNA-directed DNA polymerase activity. Both the endogenous and exogenous DNA polymerase activities exhibited up to 10-fold greater activity at the optimum concentration of manganous ion (0.025 mM for exogenous; 0.25 mM for endogenous) than at any concentration of magnesium ion ...
M R, Waite, P T, Allen
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Elimination of residual RNase activity from purified preparations of RNA-directed DNA polymerase
Biochemical and Biophysical Research Communications, 1975Abstract Preparations of RNA-directed DNA polymerase purified from RNA tumor viruses by standard methods generally contain trace amounts of single-stranded RNA endonucleolytic activity detectable only by relatively sensitive methods. This contaminating RNase activity has been found to be completely inhibited when RNA-directed DNA polymerase reactions
M S, Collett, A J, Faras
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[15b] The preparation of RNA-directed DNA polymerase from ovaries of Xenopus laevis
1974Publisher Summary This chapter describes the preparation of RNA-Directed DNA polymerase from ovaries of Xenopus laevi . Young oocytes of Xenopus laevis synthesize DNA that corresponds to a selected portion of the chromosome—the genes coding for ribosomal RNA.
Ronald D. Brown +1 more
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Biochimica et biophysica acta, 1976
Poly(vinylbenzo-18-crown-6), a water-soluble polymer endowed with ion-binding crown moieties as pendent groups, forms insoluble complexes with polyadenylate in the presence of K+; the corresponding monomeric benzo-18-crown-6, does not form a precipitate under the same conditions.
J, Pitha, J, Smid
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Poly(vinylbenzo-18-crown-6), a water-soluble polymer endowed with ion-binding crown moieties as pendent groups, forms insoluble complexes with polyadenylate in the presence of K+; the corresponding monomeric benzo-18-crown-6, does not form a precipitate under the same conditions.
J, Pitha, J, Smid
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RNA-DIRECTED DNA POLYMERASE ACTIVITY IN UNINFECTED CELLS
1974Howard M. Temin, Chil-Yong Kang
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