Results 311 to 320 of about 329,490 (335)

Polyamines stimulate natural RNA-directed DNA synthesis by Rauscher murine leukemia virus DNA polymerase

Biochemical and Biophysical Research Communications, 1981
Abstract In the presence of optimal concentrations of Mg 2+ , spermine and spermidine were found to stimulate rabbit globin mRNA-directed cDNA synthesis by Rauscher murine leukemia virus (R-MuLV) DNA polymerase. Stimulation of DNA synthesis did not occur with the polyamines putrescine or cadaverine, nor could exogenously provided salt or ammonium ...
S L, Marcus, S W, Smith, C J, Bacchi
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Innate Reverse Transcriptase Activity of DNA Polymerase for Isothermal RNA Direct Detection

Journal of the American Chemical Society, 2015
RNA detection has become one of the most robust parts in molecular biology, medical diagnostics and drug discovery. Conventional RNA detection methods involve an extra reverse transcription step, which limits their further application for RNA rapid detection.
Chao, Shi, Xiaotong, Shen, Shuyan, Niu, Cuiping, Ma   +3 more
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Cycling of RNA-directed DNA polymerase on natural and synthetic RNA templates

Nature, 1976
RNA-DIRECTED DNA polymerase (reverse transcriptase) catalyses in vivo the synthesis of DNA complementary to the RNA of tumour viruses. The DNA is integrated into the host genome and is therefore implicated in the subsequent tumorigenesis1. The mechanism by which reverse transcriptase replicates the viral RNA into complementary DNA (cDNA) and its ...
L J, Krueger, G V, Weiss, W C, Merrick, M A, Lloyd, W F, Anderson   +4 more
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Elimination of residual RNase activity from purified preparations of RNA-directed DNA polymerase

Biochemical and Biophysical Research Communications, 1975
Abstract Preparations of RNA-directed DNA polymerase purified from RNA tumor viruses by standard methods generally contain trace amounts of single-stranded RNA endonucleolytic activity detectable only by relatively sensitive methods. This contaminating RNase activity has been found to be completely inhibited when RNA-directed DNA polymerase reactions
M S, Collett, A J, Faras
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Interaction between murine type-C virus RNA-directed DNA polymerases and rifamycin derivatives

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
Abstract Some rifamycin SV derivatives are inhibitors of purified murine type-C virus RNA-directed DNA polymerase, although other polymerases are also affected. We have previously grouped these compounds into three classes (A, B, and C) based on their relative inhibitory effect on the viral polymerase.
A M, Wu, R C, Gallo
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Model RNA-directed DNA synthesis by avian myeloblastosis virus DNA polymerase and its associated RNase H

Biochemistry, 1979
A model RNA template-primer system is described for the study of RNA-directed double-stranded DNA synthesis by purified avian myeloblastosis virus DNA polymerase and its associated RNase H. In the presence of complementary RNA primer, oligo(rI), and the deoxyribonucleoside triphosphates dGTP, dTTP, and dATP, 3'-(rC)30-40-poly(rA) directs the sequential
K F, Watson, P L, Schendel, M J, Rosok, L R, Ramsey   +3 more
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Early DNA-RNA Complex from the Endogenous RNA-directed DNA Polymerase Activity of Uninfected Chicken Embryos

Nature New Biology, 1973
WHEN endogenous DNA polymerase activity was isolated from normal uninfected chicken embryos1, we suggested that it was RNA-directed for several reasons. The activity was sensitive to RNAase, but was completely resistant to DNAase and partially resistant to actinomycin D; and the DNA product of the chicken endogenous DNA polymerase activity hybridized ...
C Y, Kang, H M, Temin
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Molecular mechanisms of the RNA polymerases in plant RNA-directed DNA methylation

Trends in Biochemical Sciences
In plants, two atypical DNA-dependent RNA polymerases, RNA polymerase IV (Pol IV) and Pol V, and an RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) together produce noncoding RNAs (ncRNAs) to guide the plant-specific RNA-directed DNA methylation (RdDM). Although both Pol IV and Pol V have evolved from the canonical Pol II, they have adapted to different roles in
Guohui Xie, Xuan Du, Hongmiao Hu, Jiamu Du   +3 more
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Quantitative structure-activity correlations of rifamycins as inhibitors of viral RNA-directed DNA polymerase and mammalian .alpha. and .beta. DNA polymerases

Journal of Medicinal Chemistry, 1980
Twenty-two 3-substituted rifamycins were tested for inhibition of mammalian alpha and beta DNA polymerase and viral RNA-dependent DNA polymerase ("reverse transcriptase"). Quantitative structure--activity relationships (QSAR) were formulated for the three systems.
R S, Wu, M K, Wolpert-DeFilippes, F R, Quinn   +2 more
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[15b] The preparation of RNA-directed DNA polymerase from ovaries of Xenopus laevis

1974
Publisher Summary This chapter describes the preparation of RNA-Directed DNA polymerase from ovaries of Xenopus laevi . Young oocytes of Xenopus laevis synthesize DNA that corresponds to a selected portion of the chromosome—the genes coding for ribosomal RNA.
Ronald D. Brown, Glauco P. Tocchini-Valentini   +1 more
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