Results 51 to 60 of about 329,490 (335)

Developments in the tools and methodologies of synthetic biology. [PDF]

, 2014
Synthetic biology is principally concerned with the rational design and engineering of biologically based parts, devices, or systems. However, biological systems are generally complex and unpredictable, and are therefore, intrinsically difficult to ...
Freemont, P, Kelwick, R, MacDonald, JT, Webb, AJ   +3 more
core   +1 more source

T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis

Biotechnology & Biotechnological Equipment, 2016
Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo, Shuhui Sun, Binghua Xie, Xiao-Jing Zhu, Zhong-Min Dai   +4 more
doaj   +1 more source

Identification of Potential Biomarkers for CAD Using Integrated Expression and Methylation Data

Frontiers in Genetics, 2020
DNA methylation plays an essential role in the pathogenesis of coronary artery disease (CAD) through regulating mRNA expressions. This study aimed to identify hub genes regulated by DNA methylation as biomarkers of CAD.
Xiaokang Zhang, Yang Xiang, Dingdong He, Bin Liang, Chen Wang, Jing Luo, Fang Zheng   +6 more
doaj   +1 more source

A Pol V-mediated silencing, independent of RNA-directed DNA methylation, applies to 5S rDNA. [PDF]

PLoS Genetics, 2009
The plant-specific RNA polymerases Pol IV and Pol V are essential to RNA-directed DNA methylation (RdDM), which also requires activities from RDR2 (RNA-Dependent RNA Polymerase 2), DCL3 (Dicer-Like 3), AGO4 (Argonaute), and DRM2 (Domains Rearranged ...
Julien Douet, Sylvie Tutois, Sylvette Tourmente   +2 more
doaj   +1 more source

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase [PDF]

, 2013
RbpA is a small non-DNA-binding transcription factor that associates with RNA polymerase holoenzyme and stimulates transcription in actinobacteria, including Streptomyces coelicolor and Mycobacterium tuberculosis.
Aline Tabib-Salazar, Alting-Mees, Babcock, Bergendahl, Bierman, Bing Liu, Bougdour, Bucca, Burgess, Buttner, Campbell, Campbell, Chakraburtty, Cornilescu, Dey, England, Feklistov, Fletcher, Fontán, Forti, Gaal, Gabriel, Gruber, Haugen, Herring, Hsu, Hu, Hu, Huang, Karimova, Karimova, Kathleen O’Dwyer, Kelemen, Kelley, Kieser, Kim, Krishna, Manganelli, Manganelli, Mantsyzov, Marchant, Marie-Laure Parsy, Mark S. Paget, McClellan, Monteil, Monteil, Murakami, Newell, Otani, O’Connor, Paget, Paget, Paget, Paget, Panina, Peter J. Simpson, Philip Doughty, Pratt, Raffaelle, Richard A. Lewis, Rieping, Saecker, Sattler, Schwartz, Selmer, Sivashanmugam, Somadri Ghosh, Steve J. Matthews, Studier, Studier, Typas, van Wezel, Vassylyev, Ventura, Viollier, Vockenhuber, Wang, WHO, Young, Zhang, Zhang, Zhuo, Österberg   +82 more
core   +1 more source

Annotation of genomics data using bidirectional hidden Markov models unveils variations in Pol II transcription cycle

Molecular Systems Biology, 2014
DNA replication, transcription and repair involve the recruitment of protein complexes that change their composition as they progress along the genome in a directed or strand‐specific manner.
Benedikt Zacher, Michael Lidschreiber, Patrick Cramer, Julien Gagneur, Achim Tresch   +4 more
doaj   +1 more source

Extent of Transcription of Mouse Sarcoma-Leukemia Virus by RNA-Directed DNA Polymerase [PDF]

Proceedings of the National Academy of Sciences, 1974
The DNA product obtained from the endogenous RNA-directed DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) reaction of the Moloney sarcoma:leukemia viruses produced by the 78 A-1 cell line was analyzed and characterized.
A, Tavitian, R, Hamelin, P, Tchen, B, Olofsson, M, Boiron   +4 more
openaire   +2 more sources

Template-directed biopolymerization: tape-copying Turing machines

, 2012
DNA, RNA and proteins are among the most important macromolecules in a living cell. These molecules are polymerized by molecular machines. These natural nano-machines polymerize such macromolecules, adding one monomer at a time, using another linear ...
Chowdhury, Debashish, Sharma, Ajeet K.
core   +1 more source

A Genomic Point Mutation in the Extracellular Domain of the Thyrotropin Receptor in Patients with Graves’ Ophthalmopathy [PDF]

, 1994
Orbital and pretibial fibroblasts are targets of autoimmune attack in Graves' ophthalmopathy (GO) and pretibial dermopathy (PTD). The fibroblast autoantigen involved in these peripheral manifestations of Graves' disease and the reason for the association
Bahn, Rebecca S., Dutton, Charyl M., Heufelder, Armin E., Sarkar, Gobinda   +3 more
core   +1 more source

The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci

Genome Biology, 2017
Background The chromodomain helicase DNA-binding family of ATP-dependent chromatin remodeling factors play essential roles during eukaryote growth and development.
Rong Yang, Zhimin Zheng, Qing Chen, Lan Yang, Huan Huang, Daisuke Miki, Wenwu Wu, Liang Zeng, Jun Liu, Jin-Xing Zhou, Joe Ogas, Jian-Kang Zhu, Xin-Jian He, Heng Zhang   +13 more
doaj   +1 more source