Results 151 to 160 of about 798,183 (256)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

MALAT1 regulates mRNA processing through sequence dependent RNA-RNA and RNA-protein interactions. [PDF]

Nucleic Acids Res
Balaji A, Hall S, Johnson R, Zhu J, Ellis L, McHugh CA.   +5 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Co-profiling of in situ RNA-protein interactions and transcriptome in single cells and tissues. [PDF]

Nat Methods
Cheng QX, Xie G, Zhang X, Wang J, Ding S, Wu YX, Shi M, Duan FF, Wan ZL, Wei JJ, Xiao J, Wang Y.   +11 more
europepmc   +1 more source

RiPCA: An Assay for the Detection of RNA-Protein Interactions in Live Cells. [PDF]

Curr Protoc, 2022
Rosenblum SL, Garner AL.
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

The Plethora of RNA-Protein Interactions Model a Basis for RNA Therapies. [PDF]

Genes (Basel)
Dansereau SJ, Cui H, Dartawan RP, Sheng J.   +3 more
europepmc   +1 more source

Visualization and characterization of RNA-protein interactions in living cells. [PDF]

Nucleic Acids Res, 2021
Duan N, Arroyo M, Deng W, Cardoso MC, Leonhardt H.   +4 more
europepmc   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Single-particle studies of the effects of RNA-protein interactions on the self-assembly of RNA virus particles. [PDF]

Proc Natl Acad Sci U S A, 2022
Garmann RF, Goldfain AM, Tanimoto CR, Beren CE, Vasquez FF, Villarreal DA, Knobler CM, Gelbart WM, Manoharan VN.   +8 more
europepmc   +1 more source