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Current Opinion in Structural Biology, 1992
Abstract Recent biochemical experiments have revealed the secondary structural elements of RNA that are recognized by various RNA-binding proteins. These include structures such as hairpins, bulges, bubbles and pseudoknots that are distinct from the double-stranded form.
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Abstract Recent biochemical experiments have revealed the secondary structural elements of RNA that are recognized by various RNA-binding proteins. These include structures such as hairpins, bulges, bubbles and pseudoknots that are distinct from the double-stranded form.
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Computational Prediction of RNA-Protein Interactions
2017Experimental methods for identifying protein(s) bound by a specific promoter-associated RNA (paRNA) of interest can be expensive, difficult, and time-consuming. This chapter describes a general computational framework for identifying potential binding partners in RNA-protein complexes or RNA-protein interaction networks.
Carla M, Mann +2 more
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M(R)apping RNA–Protein Interactions
2021The systematic investigation of RNA-protein interactions is a key step towards a better understanding of the functions of RNA molecules.We developed an easy-to-use method to isolate and identify RNAs and proteins bound to long non-coding RNAs (lncRNAs ) in their native configuration.
Jasmine, Barra +2 more
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RNA-protein interactions in ribosotnes
1995Abstract In recent years, the functional role of ribosomal RNAs in protein synthesis has received a great deal of attention, as domains of highly conserved structure have been identified with the decoding site, the peptidyl transferase active centre, binding sites for the elongation factors, and other functional centres of the ribosome ...
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RNA-Protein Interaction Protocols
1999Labeling and Purification of RNA Synthesized by In Vitro Transcription, Paul A. Clarke. Joining RNA Molecules with T4 DNA Ligase, Melissa J. Moore. RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs, Michelle M. Hanna, Lori Bentsen, Michael Lucido, and Archana Sapre.
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Kinetics of RNA–protein interactions in cells
Trends in Biochemical Sciences, 2021Deepak Sharma +2 more
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Analysis of RNA–Protein Interactions by Cell Mixing
2014RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure
Sarah H, Stubbs, Nicholas K, Conrad
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Recent advances in RNA-protein interaction studies
Molecular Biology Reports, 1993The RNA binding sites for several small proteins have been characterised. These sites include double helical regions with hairpins, bulged bases and internal loops. As seen in Flock House virus structure, some proteins may recognise phosphate backbone of the canonical A-form helix not in a sequence-specific manner.
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Modulation of Noncoding RNAs (ncRNAs) and Their Potential Role as Therapeutics
Medical Epigenetics, 2021Luciano Pirola +2 more
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