Results 91 to 100 of about 591,228 (346)

PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data

Genomics, Proteomics & Bioinformatics, 2014
The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and changes in alternative splicing (AS) can impact gene function or cause disease.
Collin Tokheim, Juw Won Park, Yi Xing
doaj   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Comparison of RNA-Seq and microarray in transcriptome profiling of activated T cells.

PLoS ONE, 2014
To demonstrate the benefits of RNA-Seq over microarray in transcriptome profiling, both RNA-Seq and microarray analyses were performed on RNA samples from a human T cell activation experiment.
Shanrong Zhao, Wai-Ping Fung-Leung, Anton Bittner, Karen Ngo, Xuejun Liu   +4 more
doaj   +1 more source

Informatics for RNA sequencing: A web resource for analysis on the cloud [PDF]

, 2015
Massively parallel RNA sequencing (RNA-seq) has rapidly become the assay of choice for interrogating RNA transcript abundance and diversity. This article provides a detailed introduction to fundamental RNA-seq molecular biology and informatics concepts ...
Ainscough, Benjamin J, Griffith, Malachi, Griffith, Obi L, Spies, Nicholas C, Walker, Jason R   +4 more
core   +3 more sources

Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism

Molecular Oncology, EarlyView.
Adaptaquin selectively kills glioma stem cells while sparing differentiated brain cells. Transcriptomic and proteomic analyses show Adaptaquin disrupts iron and cholesterol homeostasis, with iron chelation amplifying cytotoxicity via cholesterol depletion, mitochondrial dysfunction, and elevated reactive oxygen species.
Adrien M. Vaquié, Davod R. Shah, Eliane E. S. Brechbühl, Michael McNicholas, Zhaoyang Xu, John H. Stockley, Laura Morcom, Diana Gold Diaz, Gemma C. Girdler, Rachel V. Seear, Gabriel Balmus, Rajiv R. Ratan, Harry Bulstrode, James A. Nathan, Manav Pathania, Kevin M. Brindle, David H. Rowitch   +16 more
wiley   +1 more source

Single-cell RNA-seq reveals the transcriptional landscape in ischemic stroke [PDF]

, 2021
Kai Zheng, Lingmin Lin, Wei Jiang, Lin Chen, Xiyue Zhang, Qian Zhang, Yi Ren, Junwei Hao   +7 more
openalex   +1 more source

MOUtbhR RNA-seq

, 2017
RNA-seq pipeline analysis of mouse single end libraries.
Manchon, Laurent, TAZI, Jamal
openaire   +1 more source

Identification of rare-disease genes using blood transcriptome sequencing and large control cohorts. [PDF]

, 2019
It is estimated that 350 million individuals worldwide suffer from rare diseases, which are predominantly caused by mutation in a single gene1. The current molecular diagnostic rate is estimated at 50%, with whole-exome sequencing (WES) among the most ...
Ashley, Euan A, Balliu, Brunilda, Battle, Alexis, Bejerano, Gill, Bernstein, Jonathan A, Bonner, Devon, Boycott, Kym M, Care4Rare Canada Consortium, Davidson, Jean M, Davis, Joe R, Ferraro, Nicole M, Fisk, Dianna G, Frésard, Laure, Grove, Megan E, Hartley, Taila, Ingelsson, Erik, Joshi, Ruchi, Kernohan, Kristin D, Kohler, Jennefer N, Li, Xin, Lind, Lars, Liu, Boxiang, Marwaha, Shruti, Merker, Jason D, Montgomery, Stephen B, Prybol, Cameron J, Reuter, Chloe M, Smail, Craig, Smith, Kevin S, Strober, Benjamin J, Teran, Nicole A, Undiagnosed Diseases Network, Utiramerur, Sowmithri, Wheeler, Matthew T, Zappala, Zachary, Zastrow, Diane B   +35 more
core  

Patient‐specific pharmacogenomics demonstrates xCT as predictive therapeutic target in colon cancer with possible implications in tumor connectivity

Molecular Oncology, EarlyView.
This study integrates transcriptomic profiling of matched tumor and healthy tissues from 32 colorectal cancer patients with functional validation in patient‐derived organoids, revealing dysregulated metabolic programs driven by overexpressed xCT (SLC7A11) and SLC3A2, identifying an oncogenic cystine/glutamate transporter signature linked to ...
Marco Strecker, Keren Zohar, Martin Böttcher, Thomas Wartmann, Henry Freudenstein, Maximilian Doelling, Mihailo Andric, Wenjie Shi, Or Kakhlon, Katrin Hippe, Beatrix Jahnke, Dimitrios Mougiakakos, Franziska Baenke, Daniel Stange, Roland S. Croner, Michal Linial, Ulf D. Kahlert   +16 more
wiley   +1 more source

Comparison of RNA-Seq and Microarray Gene Expression Platforms for the Toxicogenomic Evaluation of Liver From Short-Term Rat Toxicity Studies

Frontiers in Genetics, 2019
Gene expression profiling is a useful tool to predict and interrogate mechanisms of toxicity. RNA-Seq technology has emerged as an attractive alternative to traditional microarray platforms for conducting transcriptional profiling.
Mohan S. Rao, Terry R. Van Vleet, Rita Ciurlionis, Wayne R. Buck, Scott W. Mittelstadt, Eric A. G. Blomme, Michael J. Liguori   +6 more
doaj   +1 more source