Results 11 to 20 of about 345,547 (215)

RNA-seq reproducibility of Pseudomonas aeruginosa in laboratory models of cystic fibrosis [PDF]

Microbiology Spectrum
Reproducibility is a fundamental expectation in science and enables investigators to have confidence in their research findings and the ability to compare data from disparate sources, but evaluating reproducibility can be elusive. For example, generating
Rebecca P. Duncan, Gina R. Lewin, Daniel M. Cornforth, Frances L. Diggle, Ananya Kapur, Dina A. Moustafa, Yasmin Hilliam, Jennifer M. Bomberger, Marvin Whiteley, Joanna B. Goldberg   +9 more
doaj   +2 more sources

Holistic Optimization of Bioinformatic Analysis Pipeline for Detection and Quantification of 2′-O-Methylations in RNA by RiboMethSeq

Frontiers in Genetics, 2020
A major trend in the epitranscriptomics field over the last 5 years has been the high-throughput analysis of RNA modifications by a combination of specific chemical treatment(s), followed by library preparation and deep sequencing.
Florian Pichot, Florian Pichot, Florian Pichot, Virginie Marchand, Lilia Ayadi, Lilia Ayadi, Valérie Bourguignon-Igel, Valérie Bourguignon-Igel, Mark Helm, Yuri Motorin, Yuri Motorin   +10 more
doaj   +1 more source

Integrated analysis of single‐cell RNA‐seq dataset and bulk RNA‐seq dataset constructs a prognostic model for predicting survival in human glioblastoma

Brain and Behavior, 2022
Background Glioblastoma (GBM) is the most common primary malignant brain tumor in adults. For patients with GBM, the median overall survival (OS) is 14.6 months and the 5‐year survival rate is 7.2%. It is imperative to develop a reliable model to predict
Wenwen Lai, Defu Li, Jie Kuang, Libin Deng, Quqin Lu   +4 more
doaj   +1 more source

SeqAcademy: an educational pipeline for RNA-Seq and ChIP-Seq analysis [version 4; peer review: 2 approved, 1 not approved]

F1000Research, 2020
Quantification of gene expression and characterization of gene transcript structures are central problems in molecular biology. RNA sequencing (RNA-Seq) and chromatin immunoprecipitation sequencing (ChIP-Seq) are important methods, but can be cumbersome ...
Syed Hussain Ather, Olaitan Igbagbo Awe, Thomas J. Butler, Tamiru Denka, Stephen Andrew Semick, Wanhu Tang, Ben Busby   +6 more
doaj   +1 more source

Evaluation of methods to detect circular RNAs from single-end RNA-sequencing data

BMC Genomics, 2022
Background Circular RNA (circRNA), a class of RNA molecule with a loop structure, has recently attracted researchers due to its diverse biological functions and potential biomarkers of human diseases.
Manh Hung Nguyen, Ha-Nam Nguyen, Trung Nghia Vu   +2 more
doaj   +1 more source

Long non-coding RNA and circular RNA and coding RNA profiling of plasma exosomes of osteosarcoma by RNA seq

Scientific Data, 2023
Osteosarcoma (OS) is a primary bone tumor with high malignancy and the mechanism of hematogenous metastasis in OS is still not clear. The plasma exosomes derived from osteosarcoma play a key role in the process of tumor metastasis.
Yun Liu, Haijun Tang, Chong Li, Nenggan Huang, Jifeng Miao, Lin Chen, Kai Luo, Feicui Li, Shangyu Liu, Shijie Liao, Wenyu Feng, Xinli Zhan, Tianyu Xie, Wei Tang, Qingjun Wei, Jili Lu   +15 more
doaj   +1 more source

A map of bat virus receptors derived from single-cell multiomics

Scientific Data, 2022
Measurement(s) RNA-seq gene expression profiling assay • ATAC-Seq Technology Type(s) RNA-seq of coding RNA from single cells • Single cell ATAC-seq (cell index) Sample Characteristic - Organism Rhinolophus ...
Tianhang Lv, Xiaoshan Wang, Chao Yu, Zhifeng Wang, Rong Xiang, Linmiao Li, Yue Yuan, Yuhang Wang, Xiaoyu Wei, Yeya Yu, Xiangyang He, Libiao Zhang, Qiuting Deng, Peiying Wu, Yong Hou, Jinping Chen, Chuanyu Liu, Gary Wong, Longqi Liu   +18 more
doaj   +1 more source

RNA-Seq Transcriptomic Profiling

, 2022
This protocol describes the preparation of RNA samples from cell lines generated at CeMM for Illumina based high-throughput sequencing using polyA-enriched library preparation.
Viollet, Coralie, Santacruz, Diana, Knebel, Dagmar, Rust, Werner, Acker, Susanne, Dick, Alec   +5 more
openaire   +3 more sources

RNA-Seq Assembly – Are We There Yet? [PDF]

Frontiers in Plant Science, 2012
Transcriptomic sequence resources represent invaluable assets for research, in particular for non-model species without a sequenced genome. To date, the Next Generation Sequencing technologies 454/Roche and Illumina have been used to generate transcriptome sequence databases by mRNA-Seq for more than fifty different plant species.
Schliesky, Simon, Gowik, Udo, Weber, Andreas P. M., Bräutigam, Andrea   +3 more
openaire   +5 more sources

Unleashing the power within short-read RNA-seq for plant research: Beyond differential expression analysis and toward regulomics

Frontiers in Plant Science, 2022
RNA-seq has become a state-of-the-art technique for transcriptomic studies. Advances in both RNA-seq techniques and the corresponding analysis tools and pipelines have unprecedently shaped our understanding in almost every aspects of plant sciences ...
Min Tu, Jian Zeng, Juntao Zhang, Guozhi Fan, Guangsen Song   +4 more
doaj   +1 more source