Results 61 to 70 of about 536,139 (245)
RNA‐Seq for Bacterial Gene Expression [PDF]
AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
Poulsen, Line Dahl, Vinther, Jeppe
openaire +3 more sources
Near-optimal RNA-Seq quantification [PDF]
We present a novel approach to RNA-Seq quantification that is near optimal in speed and accuracy. Software implementing the approach, called kallisto, can be used to analyze 30 million unaligned paired-end RNA-Seq reads in less than 5 minutes on a ...
Bray, Nicolas+3 more
core +1 more source
Observation weights unlock bulk RNA-seq tools for zero inflation and single-cell applications [PDF]
Dropout events in single-cell RNA sequencing (scRNA-seq) cause many transcripts to go undetected and induce an excess of zero read counts, leading to power issues in differential expression (DE) analysis.
Clement, Lieven+8 more
core +7 more sources
COEX-Seq: Convert a Variety of Measurements of Gene Expression in RNA-Seq [PDF]
Next generation sequencing (NGS), a high-throughput DNA sequencing technology, is widely used for molecular biological studies. In NGS, RNA-sequencing (RNA-Seq), which is a short-read massively parallel sequencing, is a major quantitative transcriptome ...
Sang Cheol Kim+2 more
doaj +1 more source
Comparison of reproducibility, accuracy, sensitivity, and specificity of miRNA quantification platforms [PDF]
Given the increasing interest in their use as disease biomarkers, the establishment of reproducible, accurate, sensitive, and specific platforms for microRNA (miRNA) quantification in biofluids is of high priority.
Barczak, Andrea J+8 more
core +1 more source
Gene expression profiling is a useful tool to predict and interrogate mechanisms of toxicity. RNA-Seq technology has emerged as an attractive alternative to traditional microarray platforms for conducting transcriptional profiling.
Mohan S. Rao+6 more
doaj +1 more source
DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate
Background Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the last decade, RNA extraction remains ...
Makoto Kashima+6 more
doaj +1 more source
VIPER: Visualization Pipeline for RNA-seq, a Snakemake workflow for efficient and complete RNA-seq analysis [PDF]
RNA sequencing has become a ubiquitous technology used throughout life sciences as an effective method of measuring RNA abundance quantitatively in tissues and cells. The increase in use of RNA-seq technology has led to the continuous development of new tools for every step of analysis from alignment to downstream pathway analysis. However, effectively
Henry W. Long+15 more
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Polyribosomal RNA-Seq reveals the decreased complexity and diversity of the Arabidopsis translatome.
Recent RNA-seq studies reveal that the transcriptomes in animals and plants are more complex than previously thought, leading to the inclusion of many more splice isoforms in annotated genomes. However, it is possible that a significant proportion of the
Xingtan Zhang+4 more
doaj +1 more source
Systematic evaluation of spliced alignment programs for RNA-seq data [PDF]
High-throughput RNA sequencing is an increasingly accessible method for studying gene structure and activity on a genome-wide scale. A critical step in RNA-seq data analysis is the alignment of partial transcript reads to a reference genome sequence.
Bertone, P+10 more
core +1 more source